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Title: Polyamine regulator AMD1 promotes cell migration in epidermal wound healing
Authors: Lim, Hui Kheng
Anisa Rahim
Leo, Vonny Ivon
Das, Shatarupa
Lim, Thiam Chye
Uemura, Takeshi
Igarashi, Kazuei
Common, John
Vardy, Leah Anne
Keywords: Science::Medicine
Issue Date: 2018
Source: Lim, H. K., Anisa Rahim, Leo, V. I., Das, S., Lim, T. C., Uemura, T., . . . Vardy, L. A. (2018). Polyamine regulator AMD1 promotes cell migration in epidermal wound healing. Journal of Investigative Dermatology, 138(12), 2653-2665. doi:10.1016/j.jid.2018.05.029
Journal: Journal of Investigative Dermatology
Abstract: Wound healing is a dynamic process involving gene-expression changes that drive re-epithelialization. Here, we describe an essential role for polyamine regulator AMD1 in driving cell migration at the wound edge. The polyamines, putrescine, spermidine, and spermine are small cationic molecules that play essential roles in many cellular processes. We demonstrate that AMD1 is rapidly upregulated following wounding in human skin biopsies. Knockdown of AMD1 with small hairpin RNAs causes a delay in cell migration that is rescued by the addition of spermine. We further show that spermine can promote cell migration in keratinocytes and in human ex vivo wounds, where it significantly increases epithelial tongue migration. Knockdown of AMD1 prevents the upregulation of urokinase-type plasminogen activator/urokinase-type plasminogen activator receptor on wounding and results in a failure in actin cytoskeletal reorganization at the wound edge. We demonstrate that keratinocytes respond to wounding by modulating polyamine regulator AMD1 in order to regulate downstream gene expression and promote cell migration. This article highlights a previously unreported role for the regulation of polyamine levels and ratios in cellular behavior and fate.
ISSN: 0022-202X
DOI: 10.1016/j.jid.2018.05.029
Rights: © 2018 The Authors (published by Elsevier, Inc. on behalf of the Society for Investigative Dermatology). All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

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