Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/139897
Title: Construction of a novel catalysis system for clean and efficient preparation of Baohuoside I from Icariin based on biphase enzymatic hydrolysis
Authors: Shen, Yuping
Wang, Haiyan
Lu, Yi
Xu, Lili
Yin, Huawu
Tam, James P.
Yang, Huan
Jia, Xiaobin
Keywords: Science::Biological sciences
Issue Date: 2017
Source: Shen, Y., Wang, H., Lu, Y., Xu, L., Yin, H., Tam, J. P., . . . Jia, X. (2018). Construction of a novel catalysis system for clean and efficient preparation of Baohuoside I from Icariin based on biphase enzymatic hydrolysis. Journal of Cleaner Production, 170, 727-734. doi:10.1016/j.jclepro.2017.09.192
Journal: Journal of Cleaner Production
Abstract: Baohuoside I has better in vivo bioactivities than Icariin. Its preparation methods, such as acid hydrolysis, are of low efficiency, with undesirable byproducts. The objective of this study was to establish a novel catalysis system for its clean and efficient preparation via biphase enzymatic hydrolysis. β-glucosidase was selected from five commercial enzymes due to the best catalysis performance. After optimization of conditions, a biphase system was constructed with EtOAc and HAc-NaAc buffer (pH 4.5) (5:1, v/v) containing Icariin/β-glucosidase (3.2:1, w/w), and hydrolysis was performed at 60 °C for 2 h. Consequently, the conversion rate of Icariin was 99.92 ± 0.11% (n = 3), and the processing capacity in the system was increased from 20 mg/mL to 50 mg/mL, 2.5 times that of conventional enzymatic hydrolysis, meanwhile the procedure was much simplified. Additionally, complete hydrolysis with conversion rate at 99.14% had been achieved after the enzyme solution was used twice. The newly proposed strategy is a clean and efficient approach for the preparation of Baohuoside I and also a promising technology for producing secondary glycosides or aglycones.
URI: https://hdl.handle.net/10356/139897
ISSN: 0959-6526
DOI: 10.1016/j.jclepro.2017.09.192
Rights: © 2017 Elsevier Ltd. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

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