Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/141345
Title: Lysosome-assisted mitochondrial targeting nanoprobe based on dye-modified upconversion nanophosphors for ratiometric imaging of mitochondrial hydrogen sulfide
Authors: Li, Xiang
Zhao, Hui
Ji, Yu
Yin, Chao
Li, Jie
Yang, Zhen
Tang, Yufu
Zhang, Qichun
Fan, Quli
Huang, Wei
Keywords: Engineering::Materials
Issue Date: 2018
Source: Li, X., Zhao, H., Ji, Y., Yin, C., Li, J., Yang, Z., . . . Huang, W. (2018). Lysosome-assisted mitochondrial targeting nanoprobe based on dye-modified upconversion nanophosphors for ratiometric imaging of mitochondrial hydrogen sulfide. ACS Applied Materials & Interfaces, 10(46), 39544-39556. doi:10.1021/acsami.8b16818
Journal: ACS Applied Materials & Interfaces
Abstract: Hydrogen sulfide (H2S) is a versatile modulator in mitochondria and involved in numerous diseases caused by mitochondrial dysfunction. Therefore, many efforts have been made to develop fluorescent probes for mitochondrial H2S detection. However, these cationic small molecule probes are inapplicable for in vivo imaging because of the shallow tissue penetration and poor biostability. Herein, a ratiometric upconversion luminescence nanoprobe with an acid-activated targeting strategy is developed for detecting and bioimaging of mitochondrial H2S. The merocyanine triphenylamine-merocyanine (TPAMC)-modified upconversion nanophosphors, acting as the targeting and response component, are encapsulated into a pH-sensitive husk, composed of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy-(poly(ethylene glycol))-2000] (DSPE-PEG) and poly(l-histidine)-b-PEG, which improved the nanoprobe’s stability during transport in vivo. Under lysosomal pH, the PEG shell is interrupted and the targeting sites are exposed to further attach to mitochondria. Taking advantage of the luminescence resonance energy transfer process between TPAMC and upconversion nanophosphors, the ratiometric detection of mitochondrial H2S can be achieved with high selectivity and sensitivity. Cellular testing reveals the precise targeting to mitochondria via a lysosome delivery process. Importantly, the nanoprobe can be used for monitoring mitochondrial H2S levels in living cells and colon cancer mouse models.
URI: https://hdl.handle.net/10356/141345
ISSN: 1944-8244
DOI: 10.1021/acsami.8b16818
Rights: © 2018 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles

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