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|Title:||Retention of the structure and function of heparan sulfate biomaterials after gamma irradiation||Authors:||Smith, Raymond A. A.
Chua, R. J. E.
Carnachan, Susan M.
Tan, Clarissa L. L.
Sims, Ian M.
Hinkley, Simon F. R.
Cool, Simon M.
|Keywords:||Science::Medicine||Issue Date:||2018||Source:||Smith, R. A. A., Chua, R. J. E., Carnachan, S. M., Tan, C. L. L., Sims, I. M., Hinkley, S. F. R., . . . Cool, S. M. (2018). Retention of the structure and function of heparan sulfate biomaterials after gamma irradiation. Tissue Engineering: Part A, 24(9-10), 729-739. doi:10.1089/ten.tea.2017.0263||Journal:||Tissue Engineering: Part A||Abstract:||Heparan sulfate (HS) is a highly heterogeneous polysaccharide implicated in many important biological processes. Our previous work has demonstrated that a particular affinity-selected HS (referred to henceforth as “HS3”) is capable of enhancing the osteogenic effects of bone morphogenetic protein 2 (BMP2). Here, we gamma-irradiated HS with 26 kGy of ionizing radiation to determine how this affected the structure, composition, and function. Initial structural studies were performed on a commercial preparation of HS as a proof-of-concept. Gamma irradiation of this HS preparation did not significantly alter its structure or composition compared to nonirradiated material, as demonstrated by proton nuclear magnetic resonance spectroscopy, molecular weight analysis using size exclusion chromatography, and disaccharide compositional analysis. When HS3 was gamma irradiated, no significant effect on binding affinity toward BMP2 was observed, based on competitive surface plasmon resonance and differential scanning fluorimetry assays. Furthermore, irradiation did not significantly affect HS3's ability to synergistically enhance the osteogenic effects of BMP2 in vitro; as measured by the relative abundance of osteogenic transcripts in transdifferentiating C2C12 murine myoblasts. Additionally, no significant differences were observed in the levels of alkaline phosphatase (ALP) or calcium deposition in C2C12s treated with BMP2, together with the irradiated, or nonirradiated HS3. Irradiation of HS3 incorporated into collagen type I sponges did not affect its ability to enhance BMP2-mediated ALP expression in C2C12 cells. Our data confirm that gamma irradiation is a cost-effective and viable solution for the sterilization of HS species that allows the retention of its structure and biological function. The work suggests an effective way to incorporate clinically compatible HS species into orthotic implants, scaffolds, and other medical devices for use in the treatment of a range of diseases and disorders.||URI:||https://hdl.handle.net/10356/141441||ISSN:||1937-3341||DOI:||10.1089/ten.tea.2017.0263||Rights:||© 2018 Mary Ann Liebert, Inc. All rights reserved.||Fulltext Permission:||none||Fulltext Availability:||No Fulltext|
|Appears in Collections:||LKCMedicine Journal Articles|
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