Please use this identifier to cite or link to this item:
Title: Nanomechanical force mapping of restricted cell-to-cell collisions oscillating between contraction and relaxation
Authors: Hu, Benhui
Leow, Wan Ru
Cai, Pingqiang
Li, Yong-Qiang
Wu, Yun-Long
Chen, Xiaodong
Keywords: Engineering::Materials
Issue Date: 2017
Source: Hu, B., Leow, W. R., Cai, P., Li, Y.-Q., Wu, Y.-L., & Chen, X. (2017). Nanomechanical force mapping of restricted cell-to-cell collisions oscillating between contraction and relaxation. ACS Nano, 11(12), 12302-12310. doi:10.1021/acsnano.7b06063
Journal: ACS Nano
Abstract: Contact-mediated cell migration strongly determines the invasiveness of the corresponding cells, collective migration, and morphogenesis. The quantitative study of cellular response upon contact relies on cell-to-cell collision, which rarely occurs in conventional cell culture. Herein, we developed a strategy to activate a robust cell-to-cell collision within smooth muscle cell pairs. Nanomechanical traction force mapping reveals that the collision process is promoted by the oscillatory modulations between contraction and relaxation and orientated by the filopodial bridge composed of nanosized contractile machinery. This strategy can enhance the occurrence of cell-to-cell collision, which renders it advantageous over traditional methods that utilize micropatterned coating to confine cell pairs. Furthermore, modulation of the balance between cell tugging force and traction force can determine the repolarization of cells and thus the direction of cell migration. Overall, our approach could help to reveal the mechanistic contribution in cell motility and provide insights in tissue engineering.
ISSN: 1936-0851
DOI: 10.1021/acsnano.7b06063
Rights: © 2017 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles

Citations 10

Updated on Mar 10, 2021

Citations 10

Updated on Mar 8, 2021

Page view(s)

Updated on May 25, 2022

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.