Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/141816
Title: Matrix-assisted laser desorption ionization mass spectrometry imaging of key proteins in corneal samples from lattice dystrophy patients with TGFBI-H626R and TGFBI-R124C mutations
Authors: Venkatraman, Anandalakshmi
Hochart, Guillaume
Bonnel, David
Stauber, Jonathan
Shimmura, Shigeto
Rajamani, Lakshminaryanan
Pervushin, Konstantin
Mehta, Jodhbir S.
Keywords: Science::Biological sciences
Issue Date: 2018
Source: Venkatraman, A., Hochart, G., Bonnel, D., Stauber, J., Shimmura, S., Rajamani, L., . . . Mehta, J. S. (2019). Matrix-assisted laser desorption ionization mass spectrometry imaging of key proteins in corneal samples from lattice dystrophy patients with TGFBI-H626R and TGFBI-R124C mutations. PROTEOMICS – Clinical Applications, 13(1), 1800053-. doi:10.1002/prca.201800053
Journal: PROTEOMICS – Clinical Applications
Abstract: Scope: The purpose of this study is to identify and visualize the spatial distribution of proteins present in amyloid corneal deposits of TGFBI‐CD patients using Mass Spectrometry Imaging (MSI) and compare it with healthy control cornea. Corneal Dystrophies (CD) constitute a group of genetically inherited protein aggregation disorders that affects different layers of the cornea. With accumulated protein deposition, the cornea becomes opaque with decreased visual acuity. CD affecting the stroma and Bowman's membrane, is associated with mutations in transforming growth factor β‐induced (TGFBI ) gene. Methods: MALDI‐Mass Spectrometry Imaging (MSI) is performed on 2 patient corneas and is compared with 1 healthy control cornea using a 7T‐MALDI‐FTICR. Molecular images obtained are overlaid with congo‐red stained sections to visualize the proteins associated with the corneal amyloid aggregates. Results: MALDI‐MSI provides a relative abundance and two dimensional spatial protein signature of key proteins (TGFBIp, Apolipoprotein A‐I, Apolipoprotein A‐IV, Apolipoprotein E, Kaliocin‐1, Pyruvate Kinase and Ras related protein Rab‐10) in the patient deposits compared to the control. This is the first report of the anatomical localization of key proteins on corneal tissue section from CD patients. This may provide insight in understanding the mechanism of amyloid fibril formation in TGFBI ‐corneal dystrophy.
URI: https://hdl.handle.net/10356/141816
ISSN: 1862-8346
DOI: 10.1002/prca.201800053
Rights: © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

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