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dc.contributor.authorLim, Xin-Nien_US
dc.contributor.authorShan, Chaoen_US
dc.contributor.authorMarzinek, Jan K.en_US
dc.contributor.authorDong, Hongpingen_US
dc.contributor.authorNg, Thiam Sengen_US
dc.contributor.authorOoi, Justin Seng Geapen_US
dc.contributor.authorFibriansah, Gunturen_US
dc.contributor.authorWang, Jiaqien_US
dc.contributor.authorVerma, Chandra Shekharen_US
dc.contributor.authorBond, Peter Johnen_US
dc.contributor.authorShi, Pei-Yongen_US
dc.contributor.authorLok, Shee-meien_US
dc.identifier.citationLim, X.-N., Shan, C., Marzinek, J. K., Dong, H., Ng, T. S., Ooi, J. S. G., . . . Lok, S. (2019). Molecular basis of dengue virus serotype 2 morphological switch from 29°C to 37°C. PLOS Pathogens, 15(9), e1007996-. doi:10.1371/journal.ppat.1007996en_US
dc.description.abstractThe ability of DENV2 to display different morphologies (hence different antigenic properties) complicates vaccine and therapeutics development. Previous studies showed most strains of laboratory adapted DENV2 particles changed from smooth to "bumpy" surfaced morphology when the temperature is switched from 29°C at 37°C. Here we identified five envelope (E) protein residues different between two alternative passage history DENV2 NGC strains exhibiting smooth or bumpy surface morphologies. Several mutations performed on the smooth DENV2 infectious clone destabilized the surface, as observed by cryoEM. Molecular dynamics simulations demonstrated how chemically subtle substitution at various positions destabilized dimeric interactions between E proteins. In contrast, three out of four DENV2 clinical isolates showed a smooth surface morphology at 37°C, and only at high fever temperature (40°C) did they become "bumpy". These results imply vaccines should contain particles representing both morphologies. For prophylactic and therapeutic treatments, this study also informs on which types of antibodies should be used at different stages of an infection, i.e., those that bind to monomeric E proteins on the bumpy surface or across multiple E proteins on the smooth surfaced virus.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.relation.ispartofPLOS Pathogensen_US
dc.rights© 2019 Lim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleMolecular basis of dengue virus serotype 2 morphological switch from 29°C to 37°Cen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.organizationBioinformatics Institute, A*STARen_US
dc.description.versionPublished versionen_US
dc.subject.keywordsDengue Virusen_US
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