Please use this identifier to cite or link to this item:
Title: Involvement of organic acids and amino acids in ameliorating Ni(II) toxicity induced cell cycle dysregulation in Caulobacter crescentus : a metabolomics analysis
Authors: Jain, Abhishek
Chen, Wei Ning
Keywords: Science::Chemistry
Issue Date: 2018
Source: Jain, A., & Chen, W. N. (2018). Involvement of organic acids and amino acids in ameliorating Ni(II) toxicity induced cell cycle dysregulation in Caulobacter crescentus : a metabolomics analysis. Applied microbiology and biotechnology, 102(10), 4563–4575. doi:10.1007/s00253-018-8938-0
Journal: Applied microbiology and biotechnology
Abstract: Nickel (Ni(II)) toxicity is addressed by many different bacteria, but bacterial responses to nickel stress are still unclear. Therefore, we studied the effect of Ni(II) toxicity on cell proliferation of α-proteobacterium Caulobacter crescentus. Next, we showed the mechanism that allows C. crescentus to survive in Ni(II) stress condition. Our results revealed that the growth of C. crescentus is severely affected when the bacterium was exposed to different Ni(II) concentrations, 0.003 mM slightly affected the growth, 0.008 mM reduced the growth by 50%, and growth was completely inhibited at 0.015 mM. It was further shown that Ni(II) toxicity induced mislocalization of major regulatory proteins such as MipZ, FtsZ, ParB, and MreB, resulting in dysregulation of the cell cycle. GC-MS metabolomics analysis of Ni(II) stressed C. crescentus showed an increased level of nine important metabolites including TCA cycle intermediates and amino acids. This indicates that changes in central carbon metabolism and nitrogen metabolism are linked with the disruption of cell division process. Addition of malic acid, citric acid, alanine, proline, and glutamine to 0.015 mM Ni(II)-treated C. crescentus restored its growth. Thus, the present work shows a protective effect of these organic acids and amino acids on Ni(II) toxicity. Metabolic stimulation through the PutA/GlnA pathway, accelerated degradation of CtrA, and Ni-chelation by organic acids or amino acids are some of the possible mechanisms suggested to be involved in enhancing C. crescentus's tolerance. Our results shed light on the mechanism of increased Ni(II) tolerance in C. crescentus which may be useful in bioremediation strategies and synthetic biology applications such as the development of whole cell biosensor.
ISSN: 0175-7598
DOI: 10.1007/s00253-018-8938-0
Schools: Interdisciplinary Graduate School (IGS) 
Organisations: Advanced Environmental Biotechnology Centre
Research Centres: Nanyang Environment and Water Research Institute 
Rights: © 2018 Springer-Verlag GmbH Germany, part of Springer Nature. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:IGS Journal Articles

Citations 20

Updated on Sep 23, 2023

Web of ScienceTM
Citations 20

Updated on Sep 30, 2023

Page view(s)

Updated on Sep 30, 2023

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.