Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/142259
Title: A YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenes
Authors: Han, Sen
Shen, Danyu
Wang, Yu-Chuan
Chou, Shan-Ho
Gomelsky, Mark
Gao, Yong-Gui
Qian, Guoliang
Keywords: Science::Biological sciences
Issue Date: 2020
Source: Han, S., Shen, D., Wang, Y.-C., Chou, S.-H., Gomelsky, M., Gao, Y.-G., & Qian, G. (2020). A YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenes. Molecular Plant Pathology, 21(2), 218-229. doi:10.1111/mpp.12890
Journal: Molecular Plant Pathology
Abstract: YajQ, a binding protein of the universal bacterial second messenger cyclic di-GMP (c-di-GMP), affects virulence in several bacterial pathogens, including Xanthomonas campestris. In this bacterium, YajQ interacts with the transcription factor LysR. Upon c-di-GMP binding, the whole c-di-GMP-YajQ-LysR complex is found to dissociate from DNA, resulting in virulence gene regulation. Here, we identify a YajQ-LysR-like system in the bacterial biocontrol agent Lysobacter enzymogenes OH11 that secretes an antifungal antibiotic, heat-stable antifungal factor (HSAF) against crop fungal pathogens. We show that the YajQ homologue, CdgL (c-di-GMP receptor interacting with LysR) affects expression of the HSAF biosynthesis operon by interacting with the transcription activator LysR. The CdgL-LysR interaction enhances the apparent affinity of LysR to the promoter region upstream of the HSAF biosynthesis operon, which increases operon expression. Unlike the homologues CdgL (YajQ)-LysR system in X. campestris, we show that c-di-GMP binding to CdgL seems to weaken CdgL-LysR interactions and promote the release of CdgL from the LysR-DNA complex, which leads to decreased expression. Together, this study takes the YajQ-LysR-like system from bacterial pathogens to a crop-protecting bacterium that is able to regulate antifungal HSAF biosynthesis via disassembly of the c-di-GMP receptor-transcription activator complex.
URI: https://hdl.handle.net/10356/142259
ISSN: 1464-6722
DOI: 10.1111/mpp.12890
Rights: © 2019 The Authors. (published by British Society for Plant Pathology and John Wiley & Sons Ltd). This is an open access article under the terms of the Creative Commons Attribution NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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