Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/142259
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dc.contributor.authorHan, Senen_US
dc.contributor.authorShen, Danyuen_US
dc.contributor.authorWang, Yu-Chuanen_US
dc.contributor.authorChou, Shan-Hoen_US
dc.contributor.authorGomelsky, Marken_US
dc.contributor.authorGao, Yong-Guien_US
dc.contributor.authorQian, Guoliangen_US
dc.date.accessioned2020-06-18T02:46:45Z-
dc.date.available2020-06-18T02:46:45Z-
dc.date.issued2020-
dc.identifier.citationHan, S., Shen, D., Wang, Y.-C., Chou, S.-H., Gomelsky, M., Gao, Y.-G., & Qian, G. (2020). A YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenes. Molecular Plant Pathology, 21(2), 218-229. doi:10.1111/mpp.12890en_US
dc.identifier.issn1464-6722en_US
dc.identifier.urihttps://hdl.handle.net/10356/142259-
dc.description.abstractYajQ, a binding protein of the universal bacterial second messenger cyclic di-GMP (c-di-GMP), affects virulence in several bacterial pathogens, including Xanthomonas campestris. In this bacterium, YajQ interacts with the transcription factor LysR. Upon c-di-GMP binding, the whole c-di-GMP-YajQ-LysR complex is found to dissociate from DNA, resulting in virulence gene regulation. Here, we identify a YajQ-LysR-like system in the bacterial biocontrol agent Lysobacter enzymogenes OH11 that secretes an antifungal antibiotic, heat-stable antifungal factor (HSAF) against crop fungal pathogens. We show that the YajQ homologue, CdgL (c-di-GMP receptor interacting with LysR) affects expression of the HSAF biosynthesis operon by interacting with the transcription activator LysR. The CdgL-LysR interaction enhances the apparent affinity of LysR to the promoter region upstream of the HSAF biosynthesis operon, which increases operon expression. Unlike the homologues CdgL (YajQ)-LysR system in X. campestris, we show that c-di-GMP binding to CdgL seems to weaken CdgL-LysR interactions and promote the release of CdgL from the LysR-DNA complex, which leads to decreased expression. Together, this study takes the YajQ-LysR-like system from bacterial pathogens to a crop-protecting bacterium that is able to regulate antifungal HSAF biosynthesis via disassembly of the c-di-GMP receptor-transcription activator complex.en_US
dc.language.isoenen_US
dc.relation.ispartofMolecular Plant Pathologyen_US
dc.rights© 2019 The Authors. (published by British Society for Plant Pathology and John Wiley & Sons Ltd). This is an open access article under the terms of the Creative Commons Attribution NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleA YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenesen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doi10.1111/mpp.12890-
dc.description.versionPublished versionen_US
dc.identifier.pmid31747123-
dc.identifier.scopus2-s2.0-85075247441-
dc.identifier.issue2en_US
dc.identifier.volume21en_US
dc.identifier.spage218en_US
dc.identifier.epage229en_US
dc.subject.keywordsAntifungal Antibioticen_US
dc.subject.keywordsCdgLen_US
item.grantfulltextopen-
item.fulltextWith Fulltext-
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