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dc.contributor.authorCarissimo, Guillaumeen_US
dc.contributor.authorChan, Yi-Haoen_US
dc.contributor.authorUtt, Ageen_US
dc.contributor.authorChua, Tze-Kwangen_US
dc.contributor.authorBakar, Farhana Abuen_US
dc.contributor.authorMerits, Andresen_US
dc.contributor.authorNg, Lisa F. P.en_US
dc.identifier.citationCarissimo, G., Chan, Y.-H., Utt, A., Chua, T.-K., Bakar, F. A., Merits, A., & Ng, L. F. P. (2019). VCP/p97 is a proviral host factor for replication of chikungunya virus and other alphaviruses. Frontiers in Microbiology, 10, 2236-. doi:10.3389/fmicb.2019.02236en_US
dc.description.abstractThe evolutionarily conserved AAA+ ATPase valosin-containing protein (VCP) was previously shown to be a proviral host factor for several viruses from different viral families such as Flaviviridae, Picornaviridae, and Herpesviridae. VCP was shown to affect trafficking of Sindbis virus receptor and functions as a component of Semliki Forest virus (SFV) replicase compartment. However, the role of this cellular protein was not evaluated during replication of alphaviruses including chikungunya virus (CHIKV). Using siRNA, chemical inhibitors, and trans-replication assays, we show here that VCP is a proviral factor involved in the replication of CHIKV. Immunofluorescence assays confirmed that VCP co-localized with non-structural replicase proteins but not with dsRNA foci possibly due to VCP epitope unavailability. VCP pro-viral role is also observed with other alphaviruses such as o’nyong’nyong virus (ONNV) and SFV in different human cell lines. VCP proviral roles on several viral families now extend to replication of alphaviruses CHIKV and ONNV, emphasizing the pivotal role of VCP in virus–host interaction biology.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en_US
dc.relation.ispartofFrontiers in Microbiologyen_US
dc.rights© 2019 Carissimo, Chan, Utt, Chua, Abu Bakar, Merits and Ng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleVCP/p97 is a proviral host factor for replication of chikungunya virus and other alphavirusesen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.organizationSingapore Immunology Network, A*STARen_US
dc.description.versionPublished versionen_US
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