Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/142331
Title: VCP/p97 is a proviral host factor for replication of chikungunya virus and other alphaviruses
Authors: Carissimo, Guillaume
Chan, Yi-Hao
Utt, Age
Chua, Tze-Kwang
Bakar, Farhana Abu
Merits, Andres
Ng, Lisa F. P.
Keywords: Science::Biological sciences
Issue Date: 2019
Source: Carissimo, G., Chan, Y.-H., Utt, A., Chua, T.-K., Bakar, F. A., Merits, A., & Ng, L. F. P. (2019). VCP/p97 is a proviral host factor for replication of chikungunya virus and other alphaviruses. Frontiers in Microbiology, 10, 2236-. doi:10.3389/fmicb.2019.02236
Journal: Frontiers in Microbiology
Abstract: The evolutionarily conserved AAA+ ATPase valosin-containing protein (VCP) was previously shown to be a proviral host factor for several viruses from different viral families such as Flaviviridae, Picornaviridae, and Herpesviridae. VCP was shown to affect trafficking of Sindbis virus receptor and functions as a component of Semliki Forest virus (SFV) replicase compartment. However, the role of this cellular protein was not evaluated during replication of alphaviruses including chikungunya virus (CHIKV). Using siRNA, chemical inhibitors, and trans-replication assays, we show here that VCP is a proviral factor involved in the replication of CHIKV. Immunofluorescence assays confirmed that VCP co-localized with non-structural replicase proteins but not with dsRNA foci possibly due to VCP epitope unavailability. VCP pro-viral role is also observed with other alphaviruses such as o’nyong’nyong virus (ONNV) and SFV in different human cell lines. VCP proviral roles on several viral families now extend to replication of alphaviruses CHIKV and ONNV, emphasizing the pivotal role of VCP in virus–host interaction biology.
URI: https://hdl.handle.net/10356/142331
ISSN: 1664-302X
DOI: 10.3389/fmicb.2019.02236
Rights: © 2019 Carissimo, Chan, Utt, Chua, Abu Bakar, Merits and Ng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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