Please use this identifier to cite or link to this item:
|Title:||Biguanide-derived polymeric nanoparticles kill MRSA biofilm and suppress infection in vivo||Authors:||Li, Jianghua
Chan-Park, Mary B.
|Keywords:||Engineering::Chemical engineering||Issue Date:||2020||Source:||Li, J., Zhong, W., Zhang, K., Wang, D., Hu, J., & Chan-Park, M. B. (2020). Biguanide-derived polymeric nanoparticles kill MRSA biofilm and suppress infection in vivo. ACS Applied Materials & Interfaces, 12(19), 21231-21241. doi:10.1021/acsami.9b17747||Journal:||ACS Applied Materials & Interfaces||Abstract:||Methicillin-resistant Staphylococcus aureus (MRSA) is a significant cause of drug-resistant infections. Its propensity to develop biofilms makes it especially resistant to conventional antibiotics. We present a novel nanoparticle (NP) system made from biocompatible F-127 surfactant, tannic acid (TA), and biguanide-based polymetformin (PMET) (termed FTP NPs), which can kill MRSA biofilm bacteria effectively in vitro and in vivo and which has excellent biocompatibility. FTP NPs exhibit biofilm bactericidal activity—ability to kill bacteria both inside and outside biofilm—significantly better than many antimicrobial peptides or polymers. At low concentrations (8–32 μg/mL) in vitro, FTP NPs outperformed PMET with ∼100-fold (∼2 log10) greater reduction of MRSA USA300 biofilm bacterial cell counts, which we attribute to the antifouling property of the hydrophilic poly(ethylene glycol) contributed by F-127. Further, in an in vivo murine excisional wound model, FTP NPs achieved 1.8 log10 reduction of biofilm-associated MRSA USA300 bacteria, which significantly outperformed vancomycin (0.8 log10 reduction). Moreover, in vitro cytotoxicity tests showed that FTP NPs have less toxicity than PMET toward mammalian cells, and in vivo intravenous injection of FTP NPs at 10 mg/kg showed no acute toxicity to mice with negligible body weight loss and no significant perturbation of blood biomarkers. These biguanide-based FTP NPs are a promising approach to therapy of MRSA infections.||URI:||https://hdl.handle.net/10356/142365||ISSN:||1944-8244||DOI:||10.1021/acsami.9b17747||Rights:||This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials & Interfaces, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsami.9b17747||Fulltext Permission:||embargo_20210520||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SCBE Journal Articles|
Files in This Item:
|ACS AMI_LI JIANGHUA.pdf|
|5.2 MB||Adobe PDF||Under embargo until May 20, 2021|
Updated on Mar 10, 2021
Updated on Mar 3, 2021
Updated on Apr 10, 2021
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.