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dc.contributor.authorSu, Joshua Weimingen_US
dc.contributor.authorWang, Qiangen_US
dc.contributor.authorTian, Yaoen_US
dc.contributor.authorMadden, Leighen_US
dc.contributor.authorTeo, Erica Mei Lingen_US
dc.contributor.authorBecker, David Laurenceen_US
dc.contributor.authorLiu, Quanen_US
dc.identifier.citationSu, J. W., Wang, Q., Tian, Y., Madden, L., Teo, E. M. L., Becker, D. L., & Liu, Q. (2019). Depth sensitive Raman spectroscopy for skin wounds in rodents. Proceedings of SPIE - Clinical and Preclinical Optical Diagnostics II, 11073, 110731C -. doi:10.1117/12.2526556en_US
dc.description.abstractRaman spectroscopy has demonstrated its great potential in skin wound assessment. Given that biochemical changes in skin wound healing is a layer dependent process, depth sensitive Raman spectroscopy could enhance the power of Raman spectroscopy in this application. Considering the critical importance of rodent studies in the field of skin wound assessment, it is necessary to develop and validate a system that can perform depth sensitive measurements in rat skin with a proper target depth range. In this manuscript, we report the design, optimization and evaluation of a new snapshot depth-sensitive Raman instrument for rat skin measurements. The optical design and optimization process are presented first. The depth sensitive measurement performance is characterized on ex vivo rat skin samples with wounds. Raman signal emitted by the ex vivo rat skin from different target depths were simultaneously acquired. The feasibility of using the measured Raman spectra to differentiate between the wound edge and healthy skin was validated using PLS-LDA with leave-one-out. The accuracy of the classification improves monotonically as more data from new depths are used, which implies that each depth offers additional information useful for classification. This instrument demonstrates the ability to perform snapshot depth sensitive Raman measurements from rat skin, which paves the way towards in vivo preclinical studies of rat skin wounds.en_US
dc.rightsCopyright 2019 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.en_US
dc.titleDepth sensitive Raman spectroscopy for skin wounds in rodentsen_US
dc.typeConference Paperen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.contributor.schoolInterdisciplinary Graduate School (IGS)en_US
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en_US
dc.contributor.conferenceEuropean Conferences on Biomedical Optics 2019en_US
dc.contributor.organizationNTU Institute for Health Technologies,en_US
dc.description.versionPublished versionen_US
dc.subject.keywordsRaman Spectroscopyen_US
dc.subject.keywordsDepth Sensitiveen_US
dc.citation.conferencelocationMunich, Germanyen_US
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