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Title: Direct quantification of the translocation activities of Saccharomyces cerevisiae Pif1 helicase
Authors: Lu, Chen
Le, Shimin
Chen, Jin
Byrd, Alicia K.
Rhodes, Daniela
Raney, Kevin D.
Yan, Jie
Keywords: Science::Biological sciences
Issue Date: 2019
Source: Lu, C., Le, S., Chen, J., Byrd, A. K., Rhodes, D., Raney, K. D., & Yan, J. (2019). Direct quantification of the translocation activities of Saccharomyces cerevisiae Pif1 helicase. Nucleic Acids Research, 47(14), 7494-7501. doi:10.1093/nar/gkz541
Journal: Nucleic Acids Research
Abstract: Saccharomyces cerevisiae Pif1 (ScPif1) is known as an ATP-dependent DNA helicase that plays critical roles in a number of important biological processes such as DNA replication, telomere maintenance and genome stability maintenance. Besides its DNA helicase activity, ScPif1 is also known as a single-stranded DNA (ssDNA) translocase, while how ScPif1 translocates on ssDNA is unclear. Here, by measuring the translocation activity of individual ScPif1 molecules on ssDNA extended by mechanical force, we identified two distinct types of ssDNA translocation. In one type, ScPif1 moves along the ssDNA track with a rate of ∼140 nt/s in 100 μM ATP, whereas in the other type, ScPif1 is immobilized to a fixed location of ssDNA and generates ssDNA loops against force. Between the two, the mobile translocation is the major form at nanomolar ScPif1 concentrations although patrolling becomes more frequent at micromolar concentrations. Together, our results suggest that ScPif1 translocates on extended ssDNA in two distinct modes, primarily in a ‘mobile’ manner.
ISSN: 0305-1048
DOI: 10.1093/nar/gkz541
Schools: School of Biological Sciences 
Rights: © 2019 The Author(s). Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact
Fulltext Permission: open
Fulltext Availability: With Fulltext
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