Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/142526
Title: Effects of miR-219/miR-338 on microglia and astrocyte behaviors and astrocyte-oligodendrocyte precursor cell interactions
Authors: Nguyen, Lan Huong
Ong, William
Wang, Kai
Wang, Mingfeng
Nizetic, Dean
Chew, Sing Yian
Keywords: Engineering::Chemical engineering
Issue Date: 2019
Source: Nguyen, L. H., Ong, W., Wang, K., Wang, M., Nizetic, D. & Chew, S. Y. (2020). Effects of miR-219/miR-338 on microglia and astrocyte behaviors and astrocyte-oligodendrocyte precursor cell interactions. Neural Regeneration Research, 15(4), 739-747. doi:10.4103/1673-5374.266922
Journal: Neural Regeneration Research
Abstract: MiR-219 and miR-338 (miR-219/miR-338) are oligodendrocyte-specific microRNAs. The overexpression of these miRs in oligodendrocyte precursor cells promotes their differentiation and maturation into oligodendrocytes, which may enhance axonal remyelination after nerve injuries in the central nervous system (CNS). As such, the delivery of miR-219/miR-338 to the CNS to promote oligodendrocyte precursor cell differentiation, maturation and myelination could be a promising approach for nerve repair. However, nerve injuries in the CNS also involve other cell types, such as microglia and astrocytes. Herein, we investigated the effects of miR-219/miR-338 treatment on microglia and astrocytes in vitro and in vivo. We found that miR-219/miR-338 diminished microglial expression of pro-inflammatory cytokines and suppressed astrocyte activation. In addition, we showed that miR-219/miR-338 enhanced oligodendrocyte precursor cell differentiation and maturation in a scratch assay paradigm that re-created a nerve injury condition in vitro. Collectively, our results suggest miR-219/miR-338 as a promising treatment for axonal remyelination in the CNS following nerve injuries. All experimental procedures were approved by the Institutional Animal Care and Use Committee (IACUC), Nanyang Technological University (approval No. A0309 and A0333) on April 27, 2016 and October 8, 2016.
URI: https://hdl.handle.net/10356/142526
ISSN: 1673-5374
DOI: 10.4103/1673-5374.266922
Rights: © 2020 Neural Regeneration Research (published by Wolters Kluwer). This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution Non Commercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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