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Title: Pulsed SILAM reveals in vivo dynamics of murine brain protein translation
Authors: Ng, Ser Sue
Park, Jung Eun
Meng, Wei
Chen, Christopher P.
Kalaria, Raj N.
McCarthy, Neil E.
Sze, Siu Kwan
Keywords: Science::Biological sciences
Issue Date: 2020
Source: Ng, S. S., Park, J. E., Meng, W., Chen, C. P., Kalaria, R. N., McCarthy, N. E., & Sze, S. K. (2020). Pulsed SILAM reveals in vivo dynamics of murine brain protein translation. ACS Omega, 5(23), 13528-13540. doi:10.1021/acsomega.9b04439
Journal: ACS Omega
Abstract: Identification of proteins that are synthesized de novo in response to specific microenvironmental cues is critical for understanding molecular mechanisms that underpin vital physiological processes and pathologies. Here, we report that a brief period of SILAM (Stable Isotope Labeling of Mammals) diet enables the determination of biological functions corresponding to actively translating proteins in the mouse brain. Our results demonstrate that the synapse, dendrite, and myelin sheath are highly active neuronal structures that display rapid protein synthesis, producing key mediators of chemical signaling as well as nutrient sensing, lipid metabolism, and amyloid precursor protein processing/stability. Together, these findings confirm that protein metabolic activity varies significantly between brain functional units in vivo. Our data indicate that pulsed SILAM approaches can unravel complex protein expression dynamics in the murine brain and identify active synthetic pathways and associated functions that are likely impaired in neurodegenerative diseases.
ISSN: 2470-1343
DOI: 10.1021/acsomega.9b04439
Rights: © 2020 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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