Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/142838
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dc.contributor.authorGurbi, Biankaen_US
dc.contributor.authorBrauswetter, Diánaen_US
dc.contributor.authorVarga, Attilaen_US
dc.contributor.authorGyulavári, Pálen_US
dc.contributor.authorPénzes, Kingaen_US
dc.contributor.authorMurányi, Józsefen_US
dc.contributor.authorZámbó, Veronikaen_US
dc.contributor.authorBirtalan, Edeen_US
dc.contributor.authorKrenács, Tiboren_US
dc.contributor.authorBecker, David Laurenceen_US
dc.contributor.authorCsala, Miklósen_US
dc.contributor.authorVályi-Nagy, Istvánen_US
dc.contributor.authorPeták, Istvánen_US
dc.contributor.authorDános, Kornélen_US
dc.date.accessioned2020-07-03T07:46:05Z-
dc.date.available2020-07-03T07:46:05Z-
dc.date.issued2019-
dc.identifier.citationGurbi, B., Brauswetter, D., Varga, A., Gyulavári, P., Pénzes, K., Murányi, J., . . . Dános, K. (2019). The potential impact of Connexin 43 expression on Bcl-2 protein level and taxane sensitivity in head and neck cancers – in vitro studies. Cancers, 11(12), 1848-. doi:10.3390/cancers11121848en_US
dc.identifier.issn2072-6694en_US
dc.identifier.urihttps://hdl.handle.net/10356/142838-
dc.description.abstractThe poor prognosis of head and neck squamous cell carcinoma (HNSCC) is partly due to the lack of reliable predictive markers. Connexin 43 (Cx43) protein and its cell-communication channels have been assigned tumor suppressor functions while the anti-apoptotic Bcl-2 (B-cell lymphoma-2) protein has been associated with negative prognostic significance in cancer. This study aimed to test the role of Cx43 protein on Bcl-2 expression, tumor progression and response to taxane-based treatment in HNSCC. Human papillomavirus (HPV) negative HNSCC cell lines were tested for paclitaxel sensitivity through measuring apoptosis induction, cell viability and changes in Cx43 and Bcl-2 levels using flow cytometry, cell viability assay, immunocytochemistry and western blot. Inhibition of Cx43 expression using siRNA increased Bcl-2 protein levels in SCC25 (tongue squamous cell carcinoma) cells, while forced Cx43 expression reduced Bcl-2 levels and supported paclitaxel cytotoxicity in FaDu (hypopharynx squamous cell carcinoma) cells. In vitro results were in line with protein expression and clinicopathological features tested in tissue microarray samples of HNSCC patients. Our data demonstrate that elevated Cx43 and reduced Bcl-2 levels may indicate HNSCC sensitivity to taxane-based treatments. On the contrary, silencing of the Cx43 gene GJA1 (gap junction protein alpha-1) can result in increased Bcl-2 expression and reduced paclitaxel efficiency. Clinical tumor-based analysis also confirmed the inverse correlation between Cx43 and Bcl-2 expression.en_US
dc.language.isoenen_US
dc.relation.ispartofCancersen_US
dc.rights© 2019 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en_US
dc.subjectScience::Medicineen_US
dc.titleThe potential impact of Connexin 43 expression on Bcl-2 protein level and taxane sensitivity in head and neck cancers - in vitro studiesen_US
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en_US
dc.identifier.doi10.3390/cancers11121848-
dc.description.versionPublished versionen_US
dc.identifier.pmid31766723-
dc.identifier.scopus2-s2.0-85075477073-
dc.identifier.issue12en_US
dc.identifier.volume11en_US
dc.subject.keywordsHead and Neck Canceren_US
dc.subject.keywordsPaclitaxelen_US
item.fulltextWith Fulltext-
item.grantfulltextopen-
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