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|Title:||In vitro and in vivo evaluation of an electrospun-aligned microfibrous implant for Annulus fibrosus repair||Authors:||Gluais, Maude
Chew, Sing Yian
Le Visage, Catherine
|Keywords:||Engineering::Bioengineering||Issue Date:||2019||Source:||Gluais, M., Clouet, J., Fusellier, M., Decante, C., Moraru, C., Dutilleul, M., . . . Le Visage, C. (2019). In vitro and in vivo evaluation of an electrospun-aligned microfibrous implant for Annulus fibrosus repair. Biomaterials, 205, 81-93. doi:10.1016/j.biomaterials.2019.03.010||Journal:||Biomaterials||Abstract:||Annulus fibrosus (AF) impairment is associated with reherniation, discogenic pain, and disc degeneration after surgical partial discectomy. Due to a limited intrinsic healing capacity, defects in the AF persist over time and it is hence necessary to adopt an appropriate strategy to close and repair the damaged AF. In this study, a cell-free biodegradable scaffold made of polycaprolactone (PCL), electrospun, aligned microfibers exhibited high levels of cell colonization, alignment, and AF-like extracellular matrix deposition when evaluated in an explant culture model. The biomimetic multilayer fibrous scaffold was then assessed in an ovine model of AF impairment. After 4 weeks, no dislocation of the implants was detected, and only one sample out of six showed a partial delamination. Histological and immunohistochemical analyses revealed integration of the implant with the surrounding tissue as well as homogeneously aligned collagen fiber organization within each lamella compared to the disorganized and scarcer fibrous tissue in a randomly organized control fibrous scaffold. In conclusion, this biomimetic electrospun implant exhibited promising properties in terms of AF defect closure, with AF-like neotissue formation that fully integrated with the surrounding ovine tissue.||URI:||https://hdl.handle.net/10356/142908||ISSN:||0142-9612||DOI:||10.1016/j.biomaterials.2019.03.010||Rights:||© 2019 Elsevier Ltd. All rights reserved. This paper was published in Biomaterials and is made available with permission of Elsevier Ltd.||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SCBE Journal Articles|
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