Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/142908
Title: In vitro and in vivo evaluation of an electrospun-aligned microfibrous implant for Annulus fibrosus repair
Authors: Gluais, Maude
Clouet, Johann
Fusellier, Marion
Decante, Cyrille
Moraru, Constantin
Dutilleul, Maeva
Veziers, Joëlle
Lesoeur, Julie
Dumas, Dominique
Abadie, Jérôme
Hamel, Antoine
Bord, Eric
Chew, Sing Yian
Guicheux, Jérôme
Le Visage, Catherine
Keywords: Engineering::Bioengineering
Issue Date: 2019
Source: Gluais, M., Clouet, J., Fusellier, M., Decante, C., Moraru, C., Dutilleul, M., . . . Le Visage, C. (2019). In vitro and in vivo evaluation of an electrospun-aligned microfibrous implant for Annulus fibrosus repair. Biomaterials, 205, 81-93. doi:10.1016/j.biomaterials.2019.03.010
Journal: Biomaterials
Abstract: Annulus fibrosus (AF) impairment is associated with reherniation, discogenic pain, and disc degeneration after surgical partial discectomy. Due to a limited intrinsic healing capacity, defects in the AF persist over time and it is hence necessary to adopt an appropriate strategy to close and repair the damaged AF. In this study, a cell-free biodegradable scaffold made of polycaprolactone (PCL), electrospun, aligned microfibers exhibited high levels of cell colonization, alignment, and AF-like extracellular matrix deposition when evaluated in an explant culture model. The biomimetic multilayer fibrous scaffold was then assessed in an ovine model of AF impairment. After 4 weeks, no dislocation of the implants was detected, and only one sample out of six showed a partial delamination. Histological and immunohistochemical analyses revealed integration of the implant with the surrounding tissue as well as homogeneously aligned collagen fiber organization within each lamella compared to the disorganized and scarcer fibrous tissue in a randomly organized control fibrous scaffold. In conclusion, this biomimetic electrospun implant exhibited promising properties in terms of AF defect closure, with AF-like neotissue formation that fully integrated with the surrounding ovine tissue.
URI: https://hdl.handle.net/10356/142908
ISSN: 0142-9612
DOI: 10.1016/j.biomaterials.2019.03.010
Rights: © 2019 Elsevier Ltd. All rights reserved. This paper was published in Biomaterials and is made available with permission of Elsevier Ltd.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

Files in This Item:
File Description SizeFormat 
In vitro and in vivo ...pdf1.64 MBAdobe PDFView/Open

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.