Please use this identifier to cite or link to this item:
|Title:||Supramolecular β-sheet suckerin–based underwater adhesives||Authors:||Deepankumar, Kanagavel
De Santo, Maria P.
Hwang, Dong Soo
|Keywords:||DRNTU::Engineering::Materials||Issue Date:||2020||Source:||Deepankumar, K., Lim, C., Polte, I., Zappone, B., Labate, C., De Santo, M. P., ... Miserez, A. (2020). Supramolecular β‐sheet suckerin–based underwater adhesives. Advanced Functional Materials, 30(16), 1907534-. doi:10.1002/adfm.201907534||Journal:||Advanced Functional Materials||Abstract:||Nature has evolved several molecular strategies to ensure adhesion in aqueous environments, where artificial adhesives typically fail. One recently unveiled molecular design for wet-resistant adhesion is the cohesive cross-beta structure characteristic of amyloids, complementing the well-established surface-binding strategy of mussel adhesive proteins based on 3,4-L-Dihydroxyphenylalanine (Dopa). A family of structural proteins that self-assemble into cross beta-sheet networks are the suckerins discovered in the sucker ring teeth of squids. Here, we shed light on the wet adhesion of cross-beta motifs by producing recombinant suckerin-12, naturally lacking Dopa, and investigating its wet adhesion properties. Surprisingly, the adhesion forces measured on mica are as high as ca. 70 mN m-1, exceeding those measured for all mussel adhesive proteins to date. The pressure-sensitive adhesion of artificial suckerins is largely governed by their cross-beta motif, as evidenced using control experiments with disrupted cross-beta domains that result in almost complete loss of adhesion. We also incorporate Dopa in suckerin-12 using a residue-specific incorporation strategy that replaces Tyrosine with Dopa during expression in Escherichia coli. Although the replacement does not increase the long-term adhesion, it contributes to the initial rapid contact and enhances the adsorption onto model oxide substrates. Our findings suggest that suckerins with supramolecular cross-beta motifs are promising biopolymers for wet-resistant adhesion||URI:||https://hdl.handle.net/10356/142969||ISSN:||1616-301X||DOI:||10.1002/adfm.201907534||Rights:||© 2020 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved. This paper was published in Advanced Functional Materials and is made available with permission of WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.||Fulltext Permission:||embargo_20210427||Fulltext Availability:||With Fulltext|
|Appears in Collections:||MSE Journal Articles|
Files in This Item:
|Accepted paper||1.81 MB||Adobe PDF||Under embargo until Apr 27, 2021|
Updated on Mar 7, 2021
Updated on Mar 9, 2021
Updated on Apr 15, 2021
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.