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Title: Supramolecular β-sheet suckerin–based underwater adhesives
Authors: Deepankumar, Kanagavel
Lim, Chanoong
Polte, Ingmar
Zappone, Bruno
Labate, Cristina
De Santo, Maria P.
Mohanram, Harini
Palaniappan, Alagappan
Hwang, Dong Soo
Miserez, Ali
Keywords: DRNTU::Engineering::Materials
Issue Date: 2020
Source: Deepankumar, K., Lim, C., Polte, I., Zappone, B., Labate, C., De Santo, M. P., ... Miserez, A. (2020). Supramolecular β‐sheet suckerin–based underwater adhesives. Advanced Functional Materials, 30(16), 1907534-. doi:10.1002/adfm.201907534
Journal: Advanced Functional Materials
Abstract: Nature has evolved several molecular strategies to ensure adhesion in aqueous environments, where artificial adhesives typically fail. One recently unveiled molecular design for wet-resistant adhesion is the cohesive cross-beta structure characteristic of amyloids, complementing the well-established surface-binding strategy of mussel adhesive proteins based on 3,4-L-Dihydroxyphenylalanine (Dopa). A family of structural proteins that self-assemble into cross beta-sheet networks are the suckerins discovered in the sucker ring teeth of squids. Here, we shed light on the wet adhesion of cross-beta motifs by producing recombinant suckerin-12, naturally lacking Dopa, and investigating its wet adhesion properties. Surprisingly, the adhesion forces measured on mica are as high as ca. 70 mN m-1, exceeding those measured for all mussel adhesive proteins to date. The pressure-sensitive adhesion of artificial suckerins is largely governed by their cross-beta motif, as evidenced using control experiments with disrupted cross-beta domains that result in almost complete loss of adhesion. We also incorporate Dopa in suckerin-12 using a residue-specific incorporation strategy that replaces Tyrosine with Dopa during expression in Escherichia coli. Although the replacement does not increase the long-term adhesion, it contributes to the initial rapid contact and enhances the adsorption onto model oxide substrates. Our findings suggest that suckerins with supramolecular cross-beta motifs are promising biopolymers for wet-resistant adhesion
ISSN: 1616-301X
DOI: 10.1002/adfm.201907534
Rights: © 2020 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved. This paper was published in Advanced Functional Materials and is made available with permission of WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.
Fulltext Permission: embargo_20210427
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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