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dc.contributor.authorZhou, Yubinen_US
dc.contributor.authorWu, Yuanyuanen_US
dc.contributor.authorPokholenko, Oleksandren_US
dc.contributor.authorPapper, Vladislaven_US
dc.contributor.authorMarks, Robert S.en_US
dc.contributor.authorSteele, Terry W. J.en_US
dc.identifier.citationZhou, Y., Wu, Y., Pokholenko, O., Papper, V., Marks, R. S., & Steele, T. W. J. (2019). Design and optimisation of Photochrome Aptamer Switch Assay (PHASA). Analytica Chimica Acta, 1061, 134-141. doi:10.1016/j.aca.2019.01.049en_US
dc.description.abstractPhotochrome Aptamer Switch Assay (PHASA) is our unique and recently developed fluorescent biosensing platform that exploits the combination of aptamer adaptive binding and photoisomerisation kinetics of stilbenes. The PHASA is based on converting any suitable stilbene-ligand-aptamer complex into a reversible biosensor. Binding affinity, aptamer concentration, and stilbene molecular structure determine the PHASA performance criteria. Consequently, understanding of the structure-activity relationship of the stilbene-ligand conjugate is extremely important when predicting and optimising the biosensor sensitivity. In the present manuscript, new analytical methods relating to calculation of the limit of detection (LOD) of the PHASA are proposed and discussed. Using these methods, it is possible to optimise the independent or dependent physicochemical parameters of the stilbene-ligand conjugates and evaluate their general sensing performance. The proposed analytical methods are based on the inter-dependent relationships between the sensitivity and the range of analyte detection and provide starting conditions for known aptamer binding constants in various PHASA applications.en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.description.sponsorshipNational Research Foundation (NRF)en_US
dc.relation.ispartofAnalytica Chimica Actaen_US
dc.rights© 2019 Elsevier. All rights reserved. This paper was published in Analytica Chimica Acta and is made available with permission of Elsevier.en_US
dc.titleDesign and optimisation of Photochrome Aptamer Switch Assay (PHASA)en_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.description.versionAccepted versionen_US
dc.description.acknowledgementFunding for this publication was greatly appreciated from the Ministry of Education Tier 1 Grant RG54/13: “Photochrome Aptamer Switch Assay: A Universal Bioassay Device”. This research was also supported by the Campus for Research Excellence and Technological Enterprise (CREATE) programme (13-04-00364 А), which is supported by the National Research Foundation, Prime Minister’s Office, Singapore.en_US
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