Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/143163
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ho, James Chin Shing | en_US |
dc.contributor.author | Steininger, Christoph | en_US |
dc.contributor.author | Hiew, Shu Hui | en_US |
dc.contributor.author | Kim, Min Chul | en_US |
dc.contributor.author | Reimhult, Erik | en_US |
dc.contributor.author | Miserez, Ali | en_US |
dc.contributor.author | Cho, Nam-Joon | en_US |
dc.contributor.author | Parikh, Atul N. | en_US |
dc.contributor.author | Liedberg, Bo | en_US |
dc.date.accessioned | 2020-08-07T01:55:50Z | - |
dc.date.available | 2020-08-07T01:55:50Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Ho, J. C. S., Steininger, C., Hiew, S. H., Kim, M. C., Reimhult, E., Miserez, A., ... Liedberg, B. (2019). Minimal reconstitution of membranous web induced by a vesicle–peptide sol–gel transition. Biomacromolecules, 20(4), 1709-1718. doi:10.1021/acs.biomac.9b00081 | en_US |
dc.identifier.issn | 1525-7797 | en_US |
dc.identifier.uri | https://hdl.handle.net/10356/143163 | - |
dc.description.abstract | Positive strand RNA viruses replicate in specialized niches called membranous web within the cytoplasm of host cells. These virus replication organelles sequester viral proteins, RNA, and a variety of host factors within a fluid, amorphous matrix of clusters of endoplasmic reticulum (ER) derived vesicles. They are thought to form by the actions of a nonstructural viral protein NS4B, which remodels the ER and produces dense lipid-protein condensates. Here, we used in vitro reconstitution to identify the minimal components and elucidate physical mechanisms driving the web formation. We found that the N-terminal amphipathic domain of NS4B (peptide 4BAH2) and phospholipid vesicles (∼100-200 nm in diameter) were sufficient to produce a gel-like, viscoelastic condensate. This condensate coexists with the surrounding aqueous phase and affords rapid exchange of molecules. Together, it recapitulates the essential properties of the virus-induced membranous web. Our data support a novel phase separation mechanism in which phospholipid vesicles provide a supramolecular template spatially organizing multiple self-associating peptides thereby generating programmable multivalency de novo and inducing macroscopic phase separation. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Biomacromolecules | en_US |
dc.rights | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biomacromolecules, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.biomac.9b00081 | en_US |
dc.subject | Engineering::Chemical engineering | en_US |
dc.title | Minimal reconstitution of membranous web induced by a vesicle–peptide sol–gel transition | en_US |
dc.type | Journal Article | en |
dc.contributor.school | School of Chemical and Biomedical Engineering | en_US |
dc.contributor.school | School of Materials Science and Engineering | en_US |
dc.contributor.school | School of Biological Sciences | en_US |
dc.contributor.organization | Centre for Biomimetic Sensor Science | en_US |
dc.identifier.doi | 10.1021/acs.biomac.9b00081 | - |
dc.description.version | Accepted version | en_US |
dc.identifier.pmid | 30856330 | - |
dc.identifier.scopus | 2-s2.0-85064113333 | - |
dc.identifier.issue | 4 | en_US |
dc.identifier.volume | 20 | en_US |
dc.identifier.spage | 1709 | en_US |
dc.identifier.epage | 1718 | en_US |
dc.subject.keywords | RNA | en_US |
dc.subject.keywords | Vesicle-peptide Sol-gel Transition | en_US |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
Appears in Collections: | MSE Journal Articles |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Minimal Reconstitution of Membranous Web Induced by a Vesicle-Peptide Sol-Gel Transition.pdf | 1.38 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
50
4
Updated on Mar 23, 2024
Web of ScienceTM
Citations
50
3
Updated on Oct 27, 2023
Page view(s)
267
Updated on Mar 28, 2024
Download(s) 50
170
Updated on Mar 28, 2024
Google ScholarTM
Check
Altmetric
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.