Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/143223
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dc.contributor.authorCheong, Haolunen_US
dc.contributor.authorKim, Jisuen_US
dc.contributor.authorMu, Jingen_US
dc.contributor.authorZhang, Wenminen_US
dc.contributor.authorLi, Juanen_US
dc.contributor.authorYang, HuangHaoen_US
dc.contributor.authorXing, Bengangen_US
dc.date.accessioned2020-08-14T01:54:30Z-
dc.date.available2020-08-14T01:54:30Z-
dc.date.issued2018-
dc.identifier.citationCheong, H., Kim, J., Mu, J, Zhang, W., Li, J., Yang, H., & Xing, B. (2019). Spatiotemporal-controlled reporter for cell-surface proteolytic enzyme activity visualization. ChemBioChem, 20(4), 561-567. doi:10.1002/cbic.201800445en_US
dc.identifier.issn1439-4227en_US
dc.identifier.urihttps://hdl.handle.net/10356/143223-
dc.description.abstractLive‐cell imaging of cell‐surface‐associated proteolytic enzymes is crucial to understand their biological roles and functions in both physiological and pathological processes. However, the complexity of the cell membrane environment increases difficulties in specifically investigating targeted proteolytic activities within the microenvironment. Towards this end, a unique, photoremovable, furin‐responsive peptide probe that can undergo spatiotemporal control through UV‐light illumination has been designed and synthesized to aid in visualizing the activity of a cell‐surface‐associated protease enzyme, furin, in live cells. Prior to light irradiation, the photolabile moiety, 4,5‐dimethoxy‐2‐nitrobenzyl, in the peptide sequence of the reporter will block furin‐like enzymatic hydrolysis, and thus, no fluorescence will be observed. Upon simple light illumination, photolysis will occur, thereby revealing the uncaged peptide probe, which can undergo enzyme hydrolysis and lead to an increase in fluorescence signal; this allows the real‐time imaging of endogenous cell‐surface‐associated furin‐like enzyme function in living cells through precise spatial and temporal resolution.en_US
dc.description.sponsorshipNanyang Technological Universityen_US
dc.language.isoenen_US
dc.relation.ispartofChemBioChemen_US
dc.rightsThis is the accepted version of the following article: Cheong, H., Kim, J., Mu, J, Zhang, W., Li, J., Yang, H., & Xing, B. (2019). Spatiotemporal-controlled reporter for cell-surface proteolytic enzyme activity visualization. ChemBioChem, 20(4), 561-567. doi:10.1002/cbic.201800445, which has been published in final form at http://doi.org.remotexs.ntu.edu.sg/10.1002/cbic.201800445. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/authorresources/Journal-Authors/licensing/self-archiving.html].en_US
dc.subjectScience::Chemistryen_US
dc.titleSpatiotemporal-controlled reporter for cell-surface proteolytic enzyme activity visualizationen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen_US
dc.identifier.doi10.1002/cbic.201800445-
dc.description.versionAccepted versionen_US
dc.identifier.pmid30304583-
dc.identifier.scopus2-s2.0-85057454740-
dc.identifier.issue4en_US
dc.identifier.volume20en_US
dc.identifier.spage561en_US
dc.identifier.epage567en_US
dc.subject.keywordsBiosensorsen_US
dc.subject.keywordsEnzymesen_US
dc.description.acknowledgementThis work was partially supported by NTU-AIT-MUV NAM/16001,RG110/16 (S), RG11/13, and RG35/15; an NTU-JSPS JRP grant (M4082175.110); the Merlion program (M4082162.110) awarded by the Nanyang Technological University, Singapore; and the National Natural Science Foundation of China (NSFC; no. 51628201)en_US
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item.fulltextWith Fulltext-
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