Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/143223
Title: Spatiotemporal-controlled reporter for cell-surface proteolytic enzyme activity visualization
Authors: Cheong, Haolun
Kim, Jisu
Mu, Jing
Zhang, Wenmin
Li, Juan
Yang, HuangHao
Xing, Bengang
Keywords: Science::Chemistry
Issue Date: 2018
Source: Cheong, H., Kim, J., Mu, J, Zhang, W., Li, J., Yang, H., & Xing, B. (2019). Spatiotemporal-controlled reporter for cell-surface proteolytic enzyme activity visualization. ChemBioChem, 20(4), 561-567. doi:10.1002/cbic.201800445
Journal: ChemBioChem
Abstract: Live‐cell imaging of cell‐surface‐associated proteolytic enzymes is crucial to understand their biological roles and functions in both physiological and pathological processes. However, the complexity of the cell membrane environment increases difficulties in specifically investigating targeted proteolytic activities within the microenvironment. Towards this end, a unique, photoremovable, furin‐responsive peptide probe that can undergo spatiotemporal control through UV‐light illumination has been designed and synthesized to aid in visualizing the activity of a cell‐surface‐associated protease enzyme, furin, in live cells. Prior to light irradiation, the photolabile moiety, 4,5‐dimethoxy‐2‐nitrobenzyl, in the peptide sequence of the reporter will block furin‐like enzymatic hydrolysis, and thus, no fluorescence will be observed. Upon simple light illumination, photolysis will occur, thereby revealing the uncaged peptide probe, which can undergo enzyme hydrolysis and lead to an increase in fluorescence signal; this allows the real‐time imaging of endogenous cell‐surface‐associated furin‐like enzyme function in living cells through precise spatial and temporal resolution.
URI: https://hdl.handle.net/10356/143223
ISSN: 1439-4227
DOI: 10.1002/cbic.201800445
Rights: This is the accepted version of the following article: Cheong, H., Kim, J., Mu, J, Zhang, W., Li, J., Yang, H., & Xing, B. (2019). Spatiotemporal-controlled reporter for cell-surface proteolytic enzyme activity visualization. ChemBioChem, 20(4), 561-567. doi:10.1002/cbic.201800445, which has been published in final form at http://doi.org.remotexs.ntu.edu.sg/10.1002/cbic.201800445. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/authorresources/Journal-Authors/licensing/self-archiving.html].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SPMS Journal Articles

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