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Title: A new approach to design artificial 3D microniches with combined chemical, topographical, and rheological cues
Authors: Stoecklin, Celine
Yue, Zhang
Chen, Wilhelm W.
de Mets, Richard
Fong, Eileen
Studer, Vincent
Viasnoff, Virgile
Keywords: Engineering::Materials
Issue Date: 2018
Source: Stoecklin, C., Yue, Z., Chen, W. W., de Mets, R., Fong, E., Studer, V., & Viasnoff, V. (2018). A new approach to design artificial 3D microniches with combined chemical, topographical, and rheological cues. Advanced Biosystems, 2(7), 1700237-. doi:10.1002/adbi.201700237
Journal: Advanced Biosystems
Abstract: The in vitro methods to recapitulate environmental cues around cells are usually optimized to test a specific property of the environment (biochemical nature or the stiffness of the extracellular matrix (ECM), or nanotopography) for its capability to induce defined cell behaviors (lineage commitment, migration). Approaches that combine different environmental cues in 3D to assess the biological response of cells to the spatial organization of different biophysical and biochemical cues are growingly being developed. It is demonstrated how the lamination of through‐hole polymeric biofunctionalized membranes can be implemented to create complex bona fide microniches with differential 3D environmental properties using photoactive materials. The approach enables to create microniches ranging in size from single cells to cell aggregates. They are biofunctionalized in 3D simultaneously with topographical featured, protein patterns and structured ECM surrogate with 1 µm resolution. It is demonstrated how these niches extend in 3D the ability to pattern cells. It is exemplified how they can be used to standardize cells' shapes in 3D and to trigger the apicobasal polarization of single epithelial cells.
ISSN: 2366-7478
DOI: 10.1002/adbi.201700237
Rights: © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles

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