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dc.contributor.authorNasrollahi, Fatemehen_US
dc.contributor.authorKoh, Yun Ruien_US
dc.contributor.authorChen, Pengen_US
dc.contributor.authorVarshosaz, Jalehen_US
dc.contributor.authorKhodadadi, Abbas Alien_US
dc.contributor.authorLim, Sierinen_US
dc.identifier.citationNasrollahia, F., Koh, Y. R., Chen, P., Varshosaz, J., Khodadadi, A. A.., & Lim, S. (2019). Targeting graphene quantum dots to epidermal growth factor receptor for delivery of cisplatin and cellular imaging. Materials Science and Engineering: C, 94, 247-257. doi:10.1016/j.msec.2018.09.020en_US
dc.description.abstractThe unique properties of graphene quantum dots (GQDs) which include high loading capacity, excellent physiological stability, strong photoluminescence, biocompatibility, and facile production make them attractive nanomaterials for biomedical applications. In this work, GQDs have been explored as dual-functional targeted drug carriers and cellular bioimaging agents. The GQDs were conjugated to single chain variable fragment of antibody (scFv), which had been engineered with high affinity (B10) to epidermal growth factor receptor (EGFR), via amide covalent linkages (GQDs-scFvB10). The morphology and surface modification of GQDs were characterized by HRTEM, SDS-PAGE, FT-IR, UV-vis and fluorescence spectroscopies. Western blot analysis along with the confocal imaging of EGFR-overexpressing breast cancer cells (MDA-MB-231) demonstrated the targeting functionality of scFvB10 after conjugation to the GQDs, as well as the potential application of GQDs-scFvB10 in targeted bioimaging. The surface of targeted GQDs had a high cisplatin (CDDP) loading capacity of 50% and a pH-dependent release with slower release rate at neutral conditions, which can reduce the commonly observed systemic toxicity of CDDP. The targeted CDDP-loaded nanocarriers ((CDDP)GQDs-scFvB10) exhibited significantly higher toxicity on MDA-MB-231 cells compared to non-targeted ones suggesting their efficient uptake through EGFR. In contrast, cells with saturated EGFR showed lower uptake and cytotoxic effect of (CDDP)GQDs-scFvB10, demonstrating selectivity of the nanocarriers towards EGFR-overexpressing cells. The scFvB10-functionalized GQD is a promising platform for targeted cellular imaging and delivery of CDDP through interactions with EGFRs.en_US
dc.relation.ispartofMaterials Science and Engineering: Cen_US
dc.rights© 2018 Elsevier B.V. All rights reserved.en_US
dc.subjectEngineering::Chemical engineeringen_US
dc.titleTargeting graphene quantum dots to epidermal growth factor receptor for delivery of cisplatin and cellular imagingen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.contributor.organizationNTU-Northwestern Institute for Nanomedicineen_US
dc.subject.keywordsDrug Deliveryen_US
dc.subject.keywordsFluorescence Imagingen_US
dc.description.acknowledgementThis work was funded partially by NTU-Northwestern Institute for Nanomedicine at Nanyang Technological University, Singapore. The authors thank Arun Kumar Prabhakar for his assistance with the initial GQDs preparation, Barindra Sana and Valerie Loh for the reconstruction of the plasmid containing scFvB10 gene.en_US
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