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Title: Targeting graphene quantum dots to epidermal growth factor receptor for delivery of cisplatin and cellular imaging
Authors: Nasrollahi, Fatemeh
Koh, Yun Rui
Chen, Peng
Varshosaz, Jaleh
Khodadadi, Abbas Ali
Lim, Sierin
Keywords: Engineering::Chemical engineering
Issue Date: 2018
Source: Nasrollahia, F., Koh, Y. R., Chen, P., Varshosaz, J., Khodadadi, A. A.., & Lim, S. (2019). Targeting graphene quantum dots to epidermal growth factor receptor for delivery of cisplatin and cellular imaging. Materials Science and Engineering: C, 94, 247-257. doi:10.1016/j.msec.2018.09.020
Journal: Materials Science and Engineering: C
Abstract: The unique properties of graphene quantum dots (GQDs) which include high loading capacity, excellent physiological stability, strong photoluminescence, biocompatibility, and facile production make them attractive nanomaterials for biomedical applications. In this work, GQDs have been explored as dual-functional targeted drug carriers and cellular bioimaging agents. The GQDs were conjugated to single chain variable fragment of antibody (scFv), which had been engineered with high affinity (B10) to epidermal growth factor receptor (EGFR), via amide covalent linkages (GQDs-scFvB10). The morphology and surface modification of GQDs were characterized by HRTEM, SDS-PAGE, FT-IR, UV-vis and fluorescence spectroscopies. Western blot analysis along with the confocal imaging of EGFR-overexpressing breast cancer cells (MDA-MB-231) demonstrated the targeting functionality of scFvB10 after conjugation to the GQDs, as well as the potential application of GQDs-scFvB10 in targeted bioimaging. The surface of targeted GQDs had a high cisplatin (CDDP) loading capacity of 50% and a pH-dependent release with slower release rate at neutral conditions, which can reduce the commonly observed systemic toxicity of CDDP. The targeted CDDP-loaded nanocarriers ((CDDP)GQDs-scFvB10) exhibited significantly higher toxicity on MDA-MB-231 cells compared to non-targeted ones suggesting their efficient uptake through EGFR. In contrast, cells with saturated EGFR showed lower uptake and cytotoxic effect of (CDDP)GQDs-scFvB10, demonstrating selectivity of the nanocarriers towards EGFR-overexpressing cells. The scFvB10-functionalized GQD is a promising platform for targeted cellular imaging and delivery of CDDP through interactions with EGFRs.
ISSN: 0928-4931
DOI: 10.1016/j.msec.2018.09.020
Rights: © 2018 Elsevier B.V. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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