Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/143935
Title: One-pot dual labeling of IgG 1 and preparation of C-to-C fusion proteins through a combination of sortase A and butelase 1
Authors: Harmand, Thibault J.
Bousbaine, Djenet
Chan, Alix
Zhang, Xiaohong
Liu, David R.
Tam, James P.
Ploegh, Hidde L.
Keywords: Science::Biological sciences::Biochemistry
Issue Date: 2018
Source: Harmand, T. J., Bousbaine, D., Chan, A., Zhang, X., Liu, D. R., Tam, J. P., & Ploegh, H. L. (2018). One-pot dual labeling of IgG 1 and preparation of C-to-C fusion proteins through a combination of sortase A and butelase 1. Bioconjugate Chemistry, 29(10), 3245-3249. doi:10.1021/acs.bioconjchem.8b00563
Journal: Bioconjugate chemistry
Abstract: Site-specific chemical modification of proteins can assist in the study of their function. Furthermore, these methods are essential to develop biologicals for diagnostic and therapeutic use. Standard protein engineering protocols and recombinant expression enable the production of proteins with short peptide tags recognized by enzymes capable of site-specific modification. We report here the application of two enzymes of orthogonal specificity, sortase A and butelase 1, to prepare non-natural C-to-C fusion proteins. Using these enzymes, we further demonstrate site-selective installation of different chemical moieties at two sites in a full-size antibody molecule.
URI: https://hdl.handle.net/10356/143935
ISSN: 1520-4812
DOI: 10.1021/acs.bioconjchem.8b00563
Rights: © 2018 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

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