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https://hdl.handle.net/10356/143952
Title: | Immobilization and intracellular delivery of circular proteins by modifying a genetically incorporated unnatural amino acid | Authors: | Bi, Xiaobao Yin, Juan Hemu, Xinya Rao, Chang Tam, James P. Liu, Chuan-Fa |
Keywords: | Science::Biological sciences::Biochemistry | Issue Date: | 2018 | Source: | Bi, X., Yin, J., Hemu, X., Rao, C., Tam, J. P., & Liu, C.-F. (2018). Immobilization and intracellular delivery of circular proteins by modifying a genetically incorporated unnatural amino acid. Bioconjugate Chemistry, 29(7), 2170-2175. doi: 10.1021/acs.bioconjchem.8b00244 | Journal: | Bioconjugate Chemistry | Abstract: | Backbone-cyclic proteins are of great scientific and therapeutic interest owing to their higher stability over their linear counterparts. Modification of such cyclic proteins at a selected site would further enhance their versatility. Here we report a chemoenzymatic strategy to engineer site-selectively modified cyclic proteins by combining butelase-mediated macrocyclization with the genetic code expansion methodology. Using this strategy, we prepared a cyclic protein which was modified with biotin or a cell-penetrating peptide at a genetically incorporated noncanonical amino acid, making the cyclization-stabilized protein further amenable for site-specific immobilization and intracellular delivery. Our results point to a new avenue to engineering novel cyclic proteins with improved physicochemical and pharmacological properties for potential applications in biotechnology and medicine. | URI: | https://hdl.handle.net/10356/143952 | ISSN: | 1520-4812 | DOI: | 10.1021/acs.bioconjchem.8b00244 | Rights: | © 2018 American Chemical Society. All rights reserved. | Fulltext Permission: | none | Fulltext Availability: | No Fulltext |
Appears in Collections: | SBS Journal Articles |
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