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|Title:||Immobilization and intracellular delivery of circular proteins by modifying a genetically incorporated unnatural amino acid||Authors:||Bi, Xiaobao
Tam, James P.
|Keywords:||Science::Biological sciences::Biochemistry||Issue Date:||2018||Source:||Bi, X., Yin, J., Hemu, X., Rao, C., Tam, J. P., & Liu, C.-F. (2018). Immobilization and intracellular delivery of circular proteins by modifying a genetically incorporated unnatural amino acid. Bioconjugate Chemistry, 29(7), 2170-2175. doi: 10.1021/acs.bioconjchem.8b00244||Journal:||Bioconjugate Chemistry||Abstract:||Backbone-cyclic proteins are of great scientific and therapeutic interest owing to their higher stability over their linear counterparts. Modification of such cyclic proteins at a selected site would further enhance their versatility. Here we report a chemoenzymatic strategy to engineer site-selectively modified cyclic proteins by combining butelase-mediated macrocyclization with the genetic code expansion methodology. Using this strategy, we prepared a cyclic protein which was modified with biotin or a cell-penetrating peptide at a genetically incorporated noncanonical amino acid, making the cyclization-stabilized protein further amenable for site-specific immobilization and intracellular delivery. Our results point to a new avenue to engineering novel cyclic proteins with improved physicochemical and pharmacological properties for potential applications in biotechnology and medicine.||URI:||https://hdl.handle.net/10356/143952||ISSN:||1520-4812||DOI:||10.1021/acs.bioconjchem.8b00244||Rights:||© 2018 American Chemical Society. All rights reserved.||Fulltext Permission:||none||Fulltext Availability:||No Fulltext|
|Appears in Collections:||SBS Journal Articles|
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