Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/143952
Title: Immobilization and intracellular delivery of circular proteins by modifying a genetically incorporated unnatural amino acid
Authors: Bi, Xiaobao
Yin, Juan
Hemu, Xinya
Rao, Chang
Tam, James P.
Liu, Chuan-Fa
Keywords: Science::Biological sciences::Biochemistry
Issue Date: 2018
Source: Bi, X., Yin, J., Hemu, X., Rao, C., Tam, J. P., & Liu, C.-F. (2018). Immobilization and intracellular delivery of circular proteins by modifying a genetically incorporated unnatural amino acid. Bioconjugate Chemistry, 29(7), 2170-2175. doi: 10.1021/acs.bioconjchem.8b00244
Journal: Bioconjugate Chemistry
Abstract: Backbone-cyclic proteins are of great scientific and therapeutic interest owing to their higher stability over their linear counterparts. Modification of such cyclic proteins at a selected site would further enhance their versatility. Here we report a chemoenzymatic strategy to engineer site-selectively modified cyclic proteins by combining butelase-mediated macrocyclization with the genetic code expansion methodology. Using this strategy, we prepared a cyclic protein which was modified with biotin or a cell-penetrating peptide at a genetically incorporated noncanonical amino acid, making the cyclization-stabilized protein further amenable for site-specific immobilization and intracellular delivery. Our results point to a new avenue to engineering novel cyclic proteins with improved physicochemical and pharmacological properties for potential applications in biotechnology and medicine.
URI: https://hdl.handle.net/10356/143952
ISSN: 1520-4812
DOI: 10.1021/acs.bioconjchem.8b00244
Rights: © 2018 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

SCOPUSTM   
Citations 20

16
Updated on Jan 28, 2023

Web of ScienceTM
Citations 20

16
Updated on Jan 30, 2023

Page view(s)

196
Updated on Feb 1, 2023

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.