Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/143952
Title: Immobilization and intracellular delivery of circular proteins by modifying a genetically incorporated unnatural amino acid
Authors: Bi, Xiaobao
Yin, Juan
Hemu, Xinya
Rao, Chang
Tam, James P.
Liu, Chuan-Fa
Keywords: Science::Biological sciences::Biochemistry
Issue Date: 2018
Source: Bi, X., Yin, J., Hemu, X., Rao, C., Tam, J. P., & Liu, C.-F. (2018). Immobilization and intracellular delivery of circular proteins by modifying a genetically incorporated unnatural amino acid. Bioconjugate Chemistry, 29(7), 2170-2175. doi: 10.1021/acs.bioconjchem.8b00244
Journal: Bioconjugate Chemistry
Abstract: Backbone-cyclic proteins are of great scientific and therapeutic interest owing to their higher stability over their linear counterparts. Modification of such cyclic proteins at a selected site would further enhance their versatility. Here we report a chemoenzymatic strategy to engineer site-selectively modified cyclic proteins by combining butelase-mediated macrocyclization with the genetic code expansion methodology. Using this strategy, we prepared a cyclic protein which was modified with biotin or a cell-penetrating peptide at a genetically incorporated noncanonical amino acid, making the cyclization-stabilized protein further amenable for site-specific immobilization and intracellular delivery. Our results point to a new avenue to engineering novel cyclic proteins with improved physicochemical and pharmacological properties for potential applications in biotechnology and medicine.
URI: https://hdl.handle.net/10356/143952
ISSN: 1520-4812
DOI: 10.1021/acs.bioconjchem.8b00244
Rights: © 2018 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

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