Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/143973
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dc.contributor.authorSmith, Raymond A. A.en_US
dc.contributor.authorMurali, Sadasivamen_US
dc.contributor.authorRai, Binaen_US
dc.contributor.authorLu, Xiaohuaen_US
dc.contributor.authorLim, Zophia Xue Huien_US
dc.contributor.authorLee, Jaslyn J. L.en_US
dc.contributor.authorNurcombe, Victoren_US
dc.contributor.authorCool, Simon M.en_US
dc.date.accessioned2020-10-06T01:26:38Z-
dc.date.available2020-10-06T01:26:38Z-
dc.date.issued2018-
dc.identifier.citationSmith, R. A. A., Murali, S., Rai, B., Lu, X., Lim, Z. X. H., Lee, J. J. L., ... Cool, S. M. (2018). Minimum structural requirements for BMP-2-binding of heparin oligosaccharides. Biomaterials, 184, 41-55. doi:10.1016/j.biomaterials.2018.08.056en_US
dc.identifier.issn1878-5905en_US
dc.identifier.urihttps://hdl.handle.net/10356/143973-
dc.description.abstractBone morphogenetic proteins (BMPs) are essential during tissue repair and remodeling after injury. Glycosaminoglycan (GAG) sugars are known to enhance BMP activity in vitro and in vivo; here the interactions of BMP-2 with various glycosaminoglycan classes were compared and shown to be selective for heparin over other comparable saccharides. The minimal chain lengths and specific sulfate moieties required for heparin-derived oligosaccharide binding to BMP-2, and the ability of such oligosaccharides to promote BMP-2-induced osteogenic differentiation in vitro were then determined. BMP-2 could bind to heparin hexasaccharides (dp6) and octasaccharides (dp8), but decasaccharides (dp10) were the minimum chain length required for both efficient binding of BMP-2 and consequent heparin-dependent cell responses. N-sulfation is the most important, and 6-O-sulfation moderately important for BMP-2 binding and activity, whereas 2-O-sulfation was much less critical. Bone formation assays in vivo further confirmed that dp10, N-sulfated heparin oligosaccharides were the minimal requirement for effective enhancement of BMP-2-induced bone formation. Such information is necessary for the rational design of the next generations of heparan-based devices for bone tissue repair.en_US
dc.language.isoenen_US
dc.relation.ispartofBiomaterialsen_US
dc.rights© 2018 Elsevier Ltd. All rights reserved.en_US
dc.subjectScience::Medicineen_US
dc.titleMinimum structural requirements for BMP-2-binding of heparin oligosaccharidesen_US
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en_US
dc.identifier.doi10.1016/j.biomaterials.2018.08.056-
dc.identifier.pmid30205243-
dc.identifier.volume184en_US
dc.identifier.spage41en_US
dc.identifier.epage55en_US
dc.subject.keywordsHeparinen_US
dc.subject.keywordsGlycosaminoglycanen_US
item.fulltextNo Fulltext-
item.grantfulltextnone-
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