Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/143973
Title: Minimum structural requirements for BMP-2-binding of heparin oligosaccharides
Authors: Smith, Raymond A. A.
Murali, Sadasivam
Rai, Bina
Lu, Xiaohua
Lim, Zophia Xue Hui
Lee, Jaslyn J. L.
Nurcombe, Victor
Cool, Simon M.
Keywords: Science::Medicine
Issue Date: 2018
Source: Smith, R. A. A., Murali, S., Rai, B., Lu, X., Lim, Z. X. H., Lee, J. J. L., ... Cool, S. M. (2018). Minimum structural requirements for BMP-2-binding of heparin oligosaccharides. Biomaterials, 184, 41-55. doi:10.1016/j.biomaterials.2018.08.056
Journal: Biomaterials
Abstract: Bone morphogenetic proteins (BMPs) are essential during tissue repair and remodeling after injury. Glycosaminoglycan (GAG) sugars are known to enhance BMP activity in vitro and in vivo; here the interactions of BMP-2 with various glycosaminoglycan classes were compared and shown to be selective for heparin over other comparable saccharides. The minimal chain lengths and specific sulfate moieties required for heparin-derived oligosaccharide binding to BMP-2, and the ability of such oligosaccharides to promote BMP-2-induced osteogenic differentiation in vitro were then determined. BMP-2 could bind to heparin hexasaccharides (dp6) and octasaccharides (dp8), but decasaccharides (dp10) were the minimum chain length required for both efficient binding of BMP-2 and consequent heparin-dependent cell responses. N-sulfation is the most important, and 6-O-sulfation moderately important for BMP-2 binding and activity, whereas 2-O-sulfation was much less critical. Bone formation assays in vivo further confirmed that dp10, N-sulfated heparin oligosaccharides were the minimal requirement for effective enhancement of BMP-2-induced bone formation. Such information is necessary for the rational design of the next generations of heparan-based devices for bone tissue repair.
URI: https://hdl.handle.net/10356/143973
ISSN: 1878-5905
DOI: 10.1016/j.biomaterials.2018.08.056
Rights: © 2018 Elsevier Ltd. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:LKCMedicine Journal Articles

SCOPUSTM   
Citations 20

12
Updated on Mar 2, 2021

PublonsTM
Citations 20

12
Updated on Mar 3, 2021

Page view(s)

141
Updated on Jul 3, 2022

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.