Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/144001
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dc.contributor.authorShi, Xiangyanen_US
dc.contributor.authorPrasanna, Chinmayien_US
dc.contributor.authorPervushin, Konstantinen_US
dc.contributor.authorNordenskiöld, Larsen_US
dc.date.accessioned2020-10-07T07:09:29Z-
dc.date.available2020-10-07T07:09:29Z-
dc.date.issued2020-
dc.identifier.citationShi, X., Prasanna, C., Pervushin, K., & Nordenskiöld, L. (2020). Solid-state NMR 13C, 15N assignments of human histone H3 in the nucleosome core particle. Biomolecular NMR assignments, 14(1), 99–104. https://doi.org/10.1007/s12104-020-09927-wen_US
dc.identifier.issn1874-270Xen_US
dc.identifier.urihttps://hdl.handle.net/10356/144001-
dc.description.abstractNucleosome core particle (NCP), the basic unit of chromatin in eukaryotic cells, consists of ~ 147 bp DNA wrapped around a histone octamer (HO) formed by two H2A–H2B dimers and one (H3–H4)2 tetramer. Histones undergo various post-translational modifications (PTMs), which regulates genomic activities in different cellular phases. High-resolution structures have been solved for many nucleosomes primarily including NCPs. However, the atomic-resolution structures of nucleosome arrays and chromatin fiber, as well as the dynamics of nucleosomes remain poorly understood. Solid-state NMR (SSNMR) is one of the premier techniques to answer these questions. In this study, we present the 13C and 15N chemical shifts assignments for the globular domain of human histone H3 (hH3) using multidimensional SSNMR experiments. The obtained spectra are of outstanding resolution and the assignments are nearly 100% complete for the backbone 13C and 15N spins of R42–G132 and ~ 80% when taking into account the side chains. The secondary structure derived from the chemical shifts agrees with the previously reported X-ray crystal structure. The reported chemical shifts can be carried over to future SSNMR studies of structure and dynamics of hH3 in NCPs, nucleosome array, chromatin fibers and nucleosome-protein complexes.en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.language.isoenen_US
dc.relationMOE2018-T2-1-112en_US
dc.relationMOE2012-T3-1-001en_US
dc.relation.ispartofBiomolecular NMR Assignmentsen_US
dc.rights© 2020 Springer. This is a post-peer-review, pre-copyedit version of an article published in Biomolecular NMR Assignments. The final authenticated version is available online at: http://dx.doi.org/10.1007/s12104-020-09927-wen_US
dc.subjectScience::Biological sciencesen_US
dc.titleSolid-state NMR 13C, 15N assignments of human histone H3 in the nucleosome core particleen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doi10.1007/s12104-020-09927-w-
dc.description.versionAccepted versionen_US
dc.identifier.issue1en_US
dc.identifier.volume14en_US
dc.identifier.spage99en_US
dc.identifier.epage104en_US
dc.subject.keywordsSolid-state NMRen_US
dc.subject.keywordsHistoneen_US
dc.description.acknowledgementThis work was supported by the Singapore Ministry of Education (MOE) Academic Research Fund (AcRF) Tier 2 (MOE2018-T2-1-112) and Tier 3 grants (MOE2012-T3-1-001).en_US
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