Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/144048
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dc.contributor.authorDing, Laifengen_US
dc.contributor.authorZhang, Congcongen_US
dc.contributor.authorLiu, Zhigangen_US
dc.contributor.authorHuang, Qingxiaen_US
dc.contributor.authorZhang, Yinlongen_US
dc.contributor.authorLi, Supingen_US
dc.contributor.authorNie, Guangjunen_US
dc.contributor.authorTang, Huiruen_US
dc.contributor.authorWang, Yulanen_US
dc.date.accessioned2020-10-09T07:28:51Z-
dc.date.available2020-10-09T07:28:51Z-
dc.date.issued2020-
dc.identifier.citationDing, L., Zhang, C., Liu, Z., Huang, Q., Zhang, Y., Li, S., . . . Wang, Y. (2019). Metabonomic Investigation of Biological Effects of a New Vessel Target Protein tTF-pHLIP in a Mouse Model. Journal of Proteome Research, 19(1), 238–247. doi:10.1021/acs.jproteome.9b00507en_US
dc.identifier.issn1535-3893en_US
dc.identifier.urihttps://hdl.handle.net/10356/144048-
dc.description.abstractIn recent years, tumor microenvironment (TME) has been recognized as potential targets for tumor treatment and the tumor vascular system is one of such targets. Fusing truncated tissue factor (tTF) with pH low insertion peptides (pHLIP), tTF-pHLIP, can target tumor vessels owing to its acidic TME and cause tumor vessel occlusion by blood clotting and subsequently effectively inhibit tumor growth. To evaluate its bioeffects, we exposed the tTF-pHLIP to normal mice and mice xenograft with B16F10 tumor and analyzed the metabolic profiling of various tissues and biofluids including plasma and urine from mice treated with and without tTF-pHLIP. A combination of nuclear magnetic resonance (NMR) and gas chromatography-mass spectrometry and ultra-high-performance liquid chromatography-mass spectrometry was employed in the study. We found that tTF-pHLIP treatment can effectively reduce tumor size and concurrently ameliorate tumor-induced alterations in the TCA cycle metabolism and lipid metabolism. In addition, we found that toxicity of tTF-pHLIP to normal mice is minor and exposure of the tTF-pHLIP induced oxidative stress to the system. Hence, we concluded that tTF-pHLIP is of low toxicity and effective in reducing tumor size as well as rebalancing tumor-induced metabolic derailment.en_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Proteome Researchen_US
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Proteome Research, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jproteome.9b00507en_US
dc.subjectScience::Medicineen_US
dc.titleMetabonomic investigation of biological effects of a new vessel target protein tTF-pHLIP in a mouse modelen_US
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en_US
dc.contributor.organizationSingapore Phenome Centeren_US
dc.identifier.doi10.1021/acs.jproteome.9b00507-
dc.description.versionAccepted versionen_US
dc.identifier.pmid31603327-
dc.identifier.issue1en_US
dc.identifier.volume19en_US
dc.identifier.spage238en_US
dc.identifier.epage247en_US
dc.subject.keywordsVessel Target Proteinen_US
dc.subject.keywordsBiological Effectsen_US
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:LKCMedicine Journal Articles

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