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Title: Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects
Authors: Awasthi, Kamlesh
Change, Feng-Lin
Hsu, Hsin-Yun
Hsieh, Pei-Ying
Ohta, Nobuhiro
Keywords: Science::Chemistry
Issue Date: 2020
Source: Awasthi, K., Change, F.-L., Hsu, H.-Y., Hsieh, P.-Y., & Ohta, N. (2020). Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects. Proc. Of the 7th Asian Spectroscopy Conference (ASC 2020). doi:10.32655/ASC_8-10_Dec2020.40
Abstract: Electric field effects on dynamics and function of biological systems depend on the cell membrane capacitance and on the pulse duration of the applied electric field, which enables selective application of pulsed electric field in specific organelles and specific living cells. If electric field whose pulse width is shorter than the charging time of the outer membrane is applied to living systems, the pulsed electric field may penetrate into the cell and induce changes in intracellular function. In fact, we reported the apoptosis induced by application of nanosecond pulsed electric field (nsPEF) with a pulse width of 10–50 ns in HeLa cells.1
DOI: 10.32655/ASC_8-10_Dec2020.40
Rights: © 2020 Nanyang Technological University.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:Asian Spectroscopy Conference

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