Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/144298
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dc.contributor.authorAmmanath, Gopalen_US
dc.contributor.authorYeasmin, Sanjidaen_US
dc.contributor.authorSrinivasulu, Yuvasrien_US
dc.contributor.authorVats, Muktien_US
dc.contributor.authorCheema, Jamal Ahmeden_US
dc.contributor.authorFairuz Nabilahen_US
dc.contributor.authorSrivastava, Rohiten_US
dc.contributor.authorYildiz, Umit Hakanen_US
dc.contributor.authorAlagappan, Palaniappanen_US
dc.contributor.authorLiedberg, Boen_US
dc.date.accessioned2020-10-27T05:43:22Z-
dc.date.available2020-10-27T05:43:22Z-
dc.date.issued2019-
dc.identifier.citationAmmanath, G., Yeasmin, S., Srinivasulu, Y., Vats, M., Cheema, J. A., Fairuz Nabilah, ... Liedberg, B. (2019). Flow-through colorimetric assay for detection of nucleic acids in plasma. Analytica Chimica Acta, 1066, 102-111. doi:10.1016/j.aca.2019.03.036en_US
dc.identifier.issn0003-2670en_US
dc.identifier.urihttps://hdl.handle.net/10356/144298-
dc.description.abstractA flow-through colorimetric assay for detection of nucleic acids in plasma is reported. The proposed assay features an array of four polyvinylidene fluoride (PVDF) membranes impregnated with cationic poly (3-alkoxy-4-methylthiophene) (PT) as an optical reporter. The sensing strategy is based on monitoring the changes in optical properties of PT, upon complexation with target nucleic acids in the presence and in the absence of their corresponding complementary peptide nucleic acids (PNAs). As a proof of concept, the proposed methodology is validated using two biomarkers; lung cancer associated microRNA (mir21) and hepatitis B virus DNA (HBV-DNA). The flow-through colorimetric assay enabled detection of mir21 and HBV-DNA in plasma without requiring tedious sample pre-treatment and clean up protocols. Colorimetric responses for mir21 and HBV-DNA were obtained at nanomolar concentrations over five orders of magnitudes (from 1 nM to 10 μM), with a limit of detection of ∼0.6 nM and ∼2 nM in DI water and plasma, respectively. A logic gate system was developed to utilize the colorimetric assay responses as inputs for discrimination of mir21 and HBV-DNA and subsequently to obtain a profile of nucleic acids in samples that exceed respective clinical threshold limits, thereby enabling rapid and point of care (POC) disease diagnosis. Furthermore, the proposed methodology can be utilized for detection of a large number of nucleic acids in plasma by extending the array of PT impregnated membranes incorporated with their corresponding complementary PNAs.en_US
dc.language.isoenen_US
dc.relation.ispartofAnalytica Chimica Actaen_US
dc.rights© 2019 Elsevier B.V. All rights reserved. This paper was published in Analytica Chimica Acta and is made available with permission of Elsevier B.V.en_US
dc.subjectEngineering::Materialsen_US
dc.titleFlow-through colorimetric assay for detection of nucleic acids in plasmaen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.contributor.schoolInterdisciplinary Graduate School (IGS)en_US
dc.contributor.organizationCentre for Biomimetic Sensor Scienceen_US
dc.identifier.doi10.1016/j.aca.2019.03.036-
dc.description.versionAccepted versionen_US
dc.identifier.pmid31027524-
dc.identifier.volume1066en_US
dc.identifier.spage102en_US
dc.identifier.epage111en_US
dc.subject.keywordsColorimetric Arrayen_US
dc.subject.keywordsPolythiopheneen_US
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