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Title: CO2 -fixing liquid droplets : towards a dissection of the microalgal pyrenoid
Authors: Wunder, Tobias
Oh, Zhen Guo
Mueller-Cajar, Oliver
Keywords: Science::Biological sciences
Issue Date: 2019
Source: Wunder, T., Oh, Z. G., & Mueller‐Cajar, O. (2019). CO2 ‐fixing liquid droplets: Towards a dissection of the microalgal pyrenoid. Traffic, 20(6), 380–389. doi:10.1111/tra.12650
Journal: Traffic
Abstract: CO2 enters the biosphere via the slow, oxygen-sensitive carboxylase, Rubisco. To compensate, most microalgae saturate Rubisco with its substrate gas through a carbon dioxide concentrating mechanism. This strategy frequently involves compartmentalization of the enzyme in the pyrenoid, a non-membrane enclosed compartment of the chloroplast stroma. Recently, tremendous advances have been achieved concerning the structure, physical properties, composition and in vitro reconstitution of the pyrenoid matrix from the green alga Chlamydomonas reinhardtii. The discovery of the intrinsically disordered multivalent Rubisco linker protein EPYC1 provided a biochemical framework to explain the subsequent finding that the pyrenoid resembles a liquid droplet in vivo. Reconstitution of the corresponding liquid-liquid phase separation using pure Rubisco and EPYC1 allowed a detailed characterization of this process. Finally, a large high-quality dataset of pyrenoidal protein-protein interactions inclusive of spatial information provides ample substrate for rapid further functional dissection of the pyrenoid. Integrating and extending recent advances will inform synthetic biology efforts towards enhancing plant photosynthesis as well as contribute a versatile model towards experimentally dissecting the biochemistry of enzyme-containing membraneless organelles.
ISSN: 1398-9219
DOI: 10.1111/tra.12650
Rights: This is the accepted version of the following article: Wunder, T., Oh, Z. G., & Mueller‐Cajar, O. (2019). CO2 ‐fixing liquid droplets: Towards a dissection of the microalgal pyrenoid. Traffic, 20(6), 380–389., which has been published in final form at 10.1111/tra.12650. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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