Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/144947
Title: Synthesis of sialic acids, their derivatives, and analogs by using a whole‐cell catalyst
Authors: Lv, Xun
Cao, Hongzhi
Lin, Baixue
Wang, Wei
Zhang, Wande
Duan, Qian
Tao, Yong
Liu, Xue-Wei
Li, Xuebing
Keywords: Science::Physics
Issue Date: 2017
Source: Lv, X., Cao, H., Lin, B., Wang, W., Zhang, W., Duan, Q., . . . Li, X. (2017). Synthesis of sialic acids, their derivatives, and analogs by using a whole-cell catalyst. Chemistry - A European Journal, 23(60), 15143-15149. doi:10.1002/chem.201703083
Journal: Chemistry – A European Journal
Abstract: Sialic acids (Sias) are important constituents of cell surface glycans. Ready access to Sias in large quantities would facilitate the development of carbohydrate‐based vaccines and small‐molecule drugs. We now present a facile method for synthesizing various natural forms and non‐natural derivatives or analogs of Sias by using a whole‐cell catalyst, which is constructed by adding a plasmid containing necessary enzyme genes into a metabolically engineered strain of Escherichia coli . The flexible substrate tolerance of incorporated enzymes (N ‐acetylglucosamine 2‐epimerase and N ‐acetylneuraminic acid aldolase) allows the cellular catalyst to convert a wide range of simple and inexpensive sugars into various Sia‐related compounds through an easily scalable fermentation process. Further, syntheses using this whole‐cell biotransformation in combination with three conventional enzymatic reactions provide a series of complex Sia‐containing glycans (sialyloligosaccharides) and their derivatives bearing different substituents. The processes described herein should permit the large‐scale and economical production of both Sias and sialyloligosaccharides, and may complement existing chemical and enzymatic strategies.
URI: https://hdl.handle.net/10356/144947
ISSN: 0947-6539
DOI: 10.1002/chem.201703083
Rights: This is the accepted version of the following article: Lv, X., Cao, H., Lin, B., Wang, W., Zhang, W., Duan, Q., . . . Li, X. (2017). Synthesis of sialic acids, their derivatives, and analogs by using a whole-cell catalyst. Chemistry - A European Journal, 23(60), 15143-15149, which has been published in final form at http://dx.doi.org/10.1002/chem.201703083. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/authorresources/Journal-Authors/licensing/self-archiving.html].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SPMS Journal Articles

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