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https://hdl.handle.net/10356/144947
Title: | Synthesis of sialic acids, their derivatives, and analogs by using a whole‐cell catalyst | Authors: | Lv, Xun Cao, Hongzhi Lin, Baixue Wang, Wei Zhang, Wande Duan, Qian Tao, Yong Liu, Xue-Wei Li, Xuebing |
Keywords: | Science::Physics | Issue Date: | 2017 | Source: | Lv, X., Cao, H., Lin, B., Wang, W., Zhang, W., Duan, Q., . . . Li, X. (2017). Synthesis of sialic acids, their derivatives, and analogs by using a whole-cell catalyst. Chemistry - A European Journal, 23(60), 15143-15149. doi:10.1002/chem.201703083 | Journal: | Chemistry – A European Journal | Abstract: | Sialic acids (Sias) are important constituents of cell surface glycans. Ready access to Sias in large quantities would facilitate the development of carbohydrate‐based vaccines and small‐molecule drugs. We now present a facile method for synthesizing various natural forms and non‐natural derivatives or analogs of Sias by using a whole‐cell catalyst, which is constructed by adding a plasmid containing necessary enzyme genes into a metabolically engineered strain of Escherichia coli . The flexible substrate tolerance of incorporated enzymes (N ‐acetylglucosamine 2‐epimerase and N ‐acetylneuraminic acid aldolase) allows the cellular catalyst to convert a wide range of simple and inexpensive sugars into various Sia‐related compounds through an easily scalable fermentation process. Further, syntheses using this whole‐cell biotransformation in combination with three conventional enzymatic reactions provide a series of complex Sia‐containing glycans (sialyloligosaccharides) and their derivatives bearing different substituents. The processes described herein should permit the large‐scale and economical production of both Sias and sialyloligosaccharides, and may complement existing chemical and enzymatic strategies. | URI: | https://hdl.handle.net/10356/144947 | ISSN: | 0947-6539 | DOI: | 10.1002/chem.201703083 | Schools: | School of Physical and Mathematical Sciences | Rights: | This is the accepted version of the following article: Lv, X., Cao, H., Lin, B., Wang, W., Zhang, W., Duan, Q., . . . Li, X. (2017). Synthesis of sialic acids, their derivatives, and analogs by using a whole-cell catalyst. Chemistry - A European Journal, 23(60), 15143-15149, which has been published in final form at http://dx.doi.org/10.1002/chem.201703083. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/authorresources/Journal-Authors/licensing/self-archiving.html]. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SPMS Journal Articles |
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Synthesis of Sialic Acids, Their Derivatives, and Analogs by Using a Whole-Cell Catalyst.pdf | 1.19 MB | Adobe PDF | View/Open |
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