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dc.contributor.authorGuin, Krishnenduen_US
dc.contributor.authorChen, Yaoen_US
dc.contributor.authorMishra, Radhaen_US
dc.contributor.authorSiti Rawaidah B. M. Muzakien_US
dc.contributor.authorThimmappa, Bhagya C.en_US
dc.contributor.authorO'Brien, Caoimhe E.en_US
dc.contributor.authorButler, Geraldineen_US
dc.contributor.authorSanyal, Amartyaen_US
dc.contributor.authorSanyal, Kaustuven_US
dc.identifier.citationGuin, K., Chen, Y., Mishra, R., Siti Rawaidah B. M. Muzaki, Thimmappa, B. C., O'brien, C. E., . . . Sanyal, K. (2020). Spatial inter-centromeric interactions facilitated the emergence of evolutionary new centromeres. eLife, 9, e58556-. doi:10.7554/eLife.58556en_US
dc.description.abstractCentromeres of Candida albicans form on unique and different DNA sequences but a closely related species, Candida tropicalis, possesses homogenized inverted repeat (HIR)-associated centromeres. To investigate the mechanism of centromere type transition, we improved the fragmented genome assembly and constructed a chromosome-level genome assembly of C. tropicalis by employing PacBio sequencing, chromosome conformation capture sequencing (3C-seq), chromoblot, and genetic analysis of engineered aneuploid strains. Further, we analyzed the 3D genome organization using 3C-seq data, which revealed spatial proximity among the centromeres as well as telomeres of seven chromosomes in C. tropicalis. Intriguingly, we observed evidence of inter-centromeric translocations in the common ancestor of C. albicans and C. tropicalis. Identification of putative centromeres in closely related Candida sojae, Candida viswanathii and Candida parapsilosis indicates loss of ancestral HIR-associated centromeres and establishment of evolutionary new centromeres (ENCs) in C. albicans. We propose that spatial proximity of the homologous centromere DNA sequences facilitated karyotype rearrangements and centromere type transitions in human pathogenic yeasts of the CUG-Ser1 clade.en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.description.sponsorshipNanyang Technological Universityen_US
dc.rights© 2020 Guin et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleSpatial inter-centromeric interactions facilitated the emergence of evolutionary new centromeresen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.versionPublished versionen_US
dc.subject.keywords3D Genomeen_US
dc.subject.keywordsChromosomal Rearrangementsen_US
dc.description.acknowledgementWe thank all the members of KS laboratory and AS laboratory for stimulating discussions and critical reading of the manuscript. We acknowledge S Sun and J Heitman for helping with SMRT-seq of C. tropicalis at the PacBio sequencing facility at Duke University. We also thank AIS Khalil for helping with CNAtra software for CNV analysis. Illumina sequencing experiments for the C. sojae genome were performed at Clevergene Biocorp, Bangalore, India. We also thank B Suma for confocal microscopy, JNCASR. KG acknowledges Shyama Prasad Mukherjee Fellowship from Council of Scientific and Industrial Research (CSIR), Govt. of India [07/733 (0181)/2013-EMR-I] and financial assistance from JNCASR. This project is supported by a grant (BT/PR27490/Med/29/1323/2018) from the Department of Biotechnology (DBT), Govt. of India to KS. KS acknowledges TATA innovation fellowship (BT/HRD/35/01/03/2017) and Department of Biotechnology grant in Life Science Research, Education and Training at JNCASR (BT/INF/22/SP27679/2018). Intramural funding from JNCASR is acknowledged. This work is also supported by Nanyang Technological University’s Nanyang Assistant Professorship grant and Singapore Ministry of Education Academic Research Fund Tier 1 grant [RG39/18] to AS.en_US
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