Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/145248
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dc.contributor.authorSelão, Tiago Toscanoen_US
dc.contributor.authorJebarani, Jasminen_US
dc.contributor.authorNurul Aina Ismailen_US
dc.contributor.authorNorling, Birgittaen_US
dc.contributor.authorNixon, Peter Julianen_US
dc.date.accessioned2020-12-15T08:47:50Z-
dc.date.available2020-12-15T08:47:50Z-
dc.date.issued2020-
dc.identifier.citationSelão, T. T., Jebarani, J., Nurul Aina Ismail, Norling, B., & Nixon, P. J. (2020). Enhanced production of D-lactate in cyanobacteria by re-routing photosynthetic cyclic and pseudo-cyclic electron flow. Frontiers in Plant Science, 10, 1700-. doi:10.3389/fpls.2019.01700en_US
dc.identifier.issn1664-462Xen_US
dc.identifier.urihttps://hdl.handle.net/10356/145248-
dc.description.abstractCyanobacteria are promising chassis strains for the photosynthetic production of platform and specialty chemicals from carbon dioxide. Their efficient light harvesting and metabolic flexibility abilities have allowed a wide range of biomolecules, such as the bioplastic polylactate precursor D-lactate, to be produced, though usually at relatively low yields. In order to increase photosynthetic electron flow towards the production of D-lactate, we have generated several strains of the marine cyanobacterium Synechococcus sp. PCC 7002 (Syn7002) with deletions in genes involved in cyclic or pseudo-cyclic electron flow around photosystem I. Using a variant of the Chlamydomonas reinhardtii D-lactate dehydrogenase (LDHSRT, engineered to efficiently utilize NADPH in vivo), we have shown that deletion of either of the two flavodiiron flv homologs (involved in pseudo-cyclic electron transport) or the Syn7002 pgr5 homolog (proposed to be a vital part of the cyclic electron transport pathway) is able to increase D-lactate production in Syn7002 strains expressing LDHSRT and the Escherichia coli LldP (lactate permease), especially at low temperature (25°C) and 0.04% (v/v) CO2, though at elevated temperatures (38°C) and/or high (1%) CO2 concentrations, the effect was less obvious. The Δpgr5 background seemed to be particularly beneficial at 25°C and 0.04% (v/v) CO2, with a nearly 7-fold increase in D-lactate accumulation in comparison to the wild-type background (≈1000 vs ≈150 mg/L) and decreased side effects in comparison to the flv deletion strains. Overall, our results show that manipulation of photosynthetic electron flow is a viable strategy to increase production of platform chemicals in cyanobacteria under ambient conditions.en_US
dc.description.sponsorshipNanyang Technological Universityen_US
dc.language.isoenen_US
dc.relationM4080306en_US
dc.relationM4081714en_US
dc.relation.ispartofFrontiers in Plant Scienceen_US
dc.rights© 2020 Selão, Jebarani, Ismail, Norling and Nixon. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleEnhanced production of D-lactate in cyanobacteria by re-routing photosynthetic cyclic and pseudo-cyclic electron flowen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doi10.3389/fpls.2019.01700-
dc.description.versionPublished versionen_US
dc.identifier.pmid32117327-
dc.identifier.volume10en_US
dc.subject.keywordsCyanobacteriaen_US
dc.subject.keywordsCyclic Electron Flowen_US
dc.description.acknowledgementThis work was supported by NTU grants M4080306 to BN and M4081714 to PN.en_US
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