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Title: Antimicrobial resistance and virulence gene profiles of methicillin-resistant and - susceptible Staphylococcus aureus from food products in Denmark
Authors: Li, Heng
Andersen, Paal Skytt
Stegger, Marc
Sieber, Raphael N.
Ingmer, Hanne
Staubrand, Nicholas
Dalsgaard, Anders
Leisner, Jørgen J.
Keywords: Science::Medicine
Issue Date: 2019
Source: Li, H., Andersen, P. S., Stegger, M., Sieber, R. N., Ingmer, H., Staubrand, N., . . . Leisner, J. J. (2019). Antimicrobial resistance and virulence gene profiles of methicillin-resistant and - susceptible Staphylococcus aureus from food products in Denmark. Frontiers in Microbiology, 10, 2681-. doi:10.3389/fmicb.2019.02681
Journal: Frontiers in Microbiology
Abstract: Foods may potentially serve as vehicles for the transmission of antimicrobial-resistant variants of Staphylococcus aureus that are important in a human clinical context. Further, retail food products can be a cause of staphylococcal food poisoning. For these reasons and to account for source attribution and risk assessment, detailed information on the population structure, resistance, and virulence profiles of S. aureus originating from retail food products is necessary. In the current study, whole-genome sequences from 88 S. aureus isolates were subjected to bioinformatics analyses in relation to sequence types, antimicrobial resistance, and virulence profiles. The sequence types (ST) identified belonged to 13 clonal complexes (CC) with CC5 and CC398 being the most common. CC398 was identified as the dominant clone (n = 31). CC5 was identified as of avian origin, with the presence of φAVβ prophage genes (n = 13). In total, 39.8% of the isolates contained multiple resistance genes, and methicillin-resistant Staphylococcus aureus (MRSA) isolates were found in CC8, CC9, and CC398. Genes conferring resistance to the antimicrobial classes of β-lactams, tetracycline, and erythromycin were detected in this study, all of which are commonly used in Danish livestock production. The tst gene encoding the toxic shock syndrome toxin was for the first time identified in ST398 isolates, probably as a result of a single acquisition of a SaPI-like element. The sushi-CC398 isolates carrying the scn gene likely originated from a human reservoir, while the other isolates originated from livestock. Taken together, our results show that both human and animal reservoirs contribute to contamination in food products and that retail foods may serve as a vehicle of S. aureus between livestock and humans.
ISSN: 1664-302X
DOI: 10.3389/fmicb.2019.02681
Rights: © 2019 Li, Andersen, Stegger, Sieber, Ingmer, Staubrand, Dalsgaard and Leisner. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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