Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/145439
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dc.contributor.authorSiti Naqiah Amrunen_US
dc.contributor.authorLee, Cheryl Yi-Pinen_US
dc.contributor.authorLee, Bernetten_US
dc.contributor.authorFong, Siew-Waien_US
dc.contributor.authorYoung, Barnaby Edwarden_US
dc.contributor.authorChee, Rhonda Sin-Lingen_US
dc.contributor.authorYeo, Nicholas Kim-Wahen_US
dc.contributor.authorTorres-Ruesta, Anthonyen_US
dc.contributor.authorCarissimo, Guillaumeen_US
dc.contributor.authorPoh, Chek Mengen_US
dc.contributor.authorChang, Zi Weien_US
dc.contributor.authorTay, Matthew Ziruien_US
dc.contributor.authorChan, Yi-Haoen_US
dc.contributor.authorChen, Mark I-Chengen_US
dc.contributor.authorLow, Jenny Guek-Hongen_US
dc.contributor.authorTambyah, Paul A.en_US
dc.contributor.authorKalimuddin, Shirinen_US
dc.contributor.authorPada, Surinderen_US
dc.contributor.authorTan, Seow-Yenen_US
dc.contributor.authorSun, Louisa Jinen_US
dc.contributor.authorLeo, Yee-Sinen_US
dc.contributor.authorLye, David C.en_US
dc.contributor.authorRenia, Laurenten_US
dc.contributor.authorNg, Lisa F. P.en_US
dc.date.accessioned2020-12-21T09:13:13Z-
dc.date.available2020-12-21T09:13:13Z-
dc.date.issued2020-
dc.identifier.citationSiti Naqiah Amrun, Lee, C. Y.-P., Lee, B., Fong, S.-W., Young, B. E., Chee, R. S.-L., . . . Ng, L. F. P. (2020). Linear B-cell epitopes in the spike and nucleocapsid proteins as markers of SARS-CoV-2 exposure and disease severity. EBioMedicine, 58, 102911-. doi:10.1016/j.ebiom.2020.102911en_US
dc.identifier.issn2352-3964en_US
dc.identifier.urihttps://hdl.handle.net/10356/145439-
dc.description.abstractBackground: Given the unceasing worldwide surge in COVID-19 cases, there is an imperative need to develop highly specific and sensitive serology assays to define exposure to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Methods: Pooled plasma samples from PCR positive COVID-19 patients were used to identify linear B-cell epitopes from a SARS-CoV-2 peptide library of spike (S), envelope (E), membrane (M), and nucleocapsid (N) structural proteins by peptide-based ELISA. Hit epitopes were further validated with 79 COVID-19 patients with different disease severity status, 13 seasonal human CoV, 20 recovered SARS patients and 22 healthy donors. Findings: Four immunodominant epitopes, S14P5, S20P2, S21P2 and N4P5, were identified on the S and N viral proteins. IgG responses to all identified epitopes displayed a strong detection profile, with N4P5 achieving the highest level of specificity (100%) and sensitivity (>96%) against SARS-CoV-2. Furthermore, the magnitude of IgG responses to S14P5, S21P2 and N4P5 were strongly associated with disease severity. Interpretation: IgG responses to the peptide epitopes can serve as useful indicators for the degree of immunopathology in COVID-19 patients, and function as higly specific and sensitive sero-immunosurveillance tools for recent or past SARS-CoV-2 infections. The flexibility of these epitopes to be used alone or in combination will allow for the development of improved point-of-care-tests (POCTs).en_US
dc.description.sponsorshipAgency for Science, Technology and Research (A*STAR)en_US
dc.description.sponsorshipNational Medical Research Council (NMRC)en_US
dc.language.isoenen_US
dc.relationCOVID19RF-001en_US
dc.relationCCGSFPOR20002en_US
dc.relation.ispartofEBioMedicineen_US
dc.rights© 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license. (http://creativecommons.org/licenses/by-nc-nd/4.0/)en_US
dc.subjectScience::Medicineen_US
dc.titleLinear B-cell epitopes in the spike and nucleocapsid proteins as markers of SARS-CoV-2 exposure and disease severityen_US
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en_US
dc.identifier.doi10.1016/j.ebiom.2020.102911-
dc.description.versionPublished versionen_US
dc.identifier.pmid32711254-
dc.identifier.volume58en_US
dc.subject.keywordsEpitopesen_US
dc.subject.keywordsSARS-CoV-2en_US
dc.description.acknowledgementBiomedical Research Council (BMRC), the A*ccelerate GAP-funded project (ACCL/19-GAP064-R20H-H) from Agency of Science, Technology and Research (A*STAR), and National Medical Research Council (NMRC) COVID-19 Research fund (COVID19RF-001) and CCGSFPOR20002. ATR is supported by the Singapore International Graduate Award (SINGA), A*STAR.en_US
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