Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/145542
Title: Exonuclease III-regulated target cyclic amplification-based single nucleotide polymorphism detection using ultrathin ternary chalcogenide nanosheets
Authors: Hu, Yanling
Tan, Chaoliang
Lin, Xin
Lai, Zhuangchai
Zhang, Xiao
Lu, Qipeng
Feng, Ning
Yang, Dongliang
Weng, Lixing
Keywords: Science::Chemistry
Issue Date: 2019
Source: Hu, Y., Tan, C., Lin, X., Lai, Z., Zhang, X., Lu, Q., . . . Weng, L. (2019). Exonuclease III-regulated target cyclic amplification-based single nucleotide polymorphism detection using ultrathin ternary chalcogenide nanosheets. Frontiers in Chemistry, 7, 844-. doi:10.3389/fchem.2019.00844
Journal: Frontiers in Chemistry 
Abstract: Herein, we report that the ternary chalcogenide nanosheet exhibits different affinity toward oligonucleotides with different lengths and efficiently quenches the fluorescence of dye-labeled DNA probes. Based on these findings, as a proof-of-concept application, the ternary chalcogenide nanosheet is used as a target cyclic amplification biosensor, showing high specificity in discriminating single-base mismatch. This simple strategy is fast and sensitive for the single nucleotide polymorphism detection. Ultralow detection limit of unlabeled target (250 fM) and high discrimination ratio (5%) in the mixture of perfect match (mutant-type) and single-base mismatch (wild-type) target are achieved. This sensing method is extensively compatible for the single nucleotide polymorphism detection in clinical samples, making it a promising tool for the mutation-based clinical diagnostic and genomic research.
URI: https://hdl.handle.net/10356/145542
ISSN: 2296-2646
DOI: 10.3389/fchem.2019.00844
Rights: © 2019 Hu, Tan, Lin, Lai, Zhang, Lu, Feng, Yang and Weng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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