Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/145606
Title: Impaired nuclear export of the ribonucleoprotein complex and virus-induced cytotoxicity combine to restrict propagation of the A/Duck/Malaysia/02/2001 (H9N2) virus in human airway cells
Authors: Kumar, Sriram
Yeo, Dawn
Muralidharan, Nisha Harur
Lai, Soak-Kuan
Tong, Cathlyn
Tan, Boon Huan
Sugrue, Richard J.
Keywords: Science::Biological sciences
Issue Date: 2020
Source: Kumar, S., Yeo, D., Muralidharan, N. H., Lai, S.-K., Tong, C., Tan, B. H. & Sugrue, R. J. Impaired nuclear export of the ribonucleoprotein complex and virus-induced cytotoxicity combine to restrict propagation of the A/Duck/Malaysia/02/2001 (H9N2) virus in human airway cells. Cells, 9(2), 355-. doi:10.3390/cells9020355
Project: NMRC/ H1N1R/002/2009
2012-T1-001–157
Journal: Cells
Abstract: In humans, (A549) cells impaired H9N2 virus nuclear export of the ribonucleoprotein (RNP) complex contrasted with the early and efficient nuclear export of the H1N1/WSN and pH1N1 virus RNP complexes. Although nuclear export of the RNP complex occurred via the nuclear pore complex, H9N2 virus infection also induced modifications in the nuclear envelope and induced cell cytotoxicity. Reduced PA protein levels in H9N2 virus-infected A549 cells occurred, and this phenomenon was independent of virus infection. Silencing the H1N1/WSN PA protein expression leads to impaired nuclear export of RNP complexes, suggesting that the impaired nuclear export of the H9N2 virus RNP complex may be one of the consequences of reduced PA protein levels. Early and efficient export of the RNP complex occurred in H9N2 virus-infected avian (CEF) cells, although structural changes in the nuclear envelope also occurred. Collectively our data suggest that a combination of delayed nuclear export and virus-induced cell cytotoxicity restricts H9N2 virus transmission in A549 cells. However, the early and efficient export of the RNP complex mitigated the effects of virus-induced cytotoxicity on H9N2 virus transmission in CEF cells. Our findings highlight the multi-factorial nature of host-adaptation of the polymerase proteins of avian influenza viruses in non-avian cell environments.
URI: https://hdl.handle.net/10356/145606
ISSN: 2073-4409
DOI: 10.3390/cells9020355
Rights: © 2020 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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