Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/145615
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dc.contributor.authorTran, Tuan Minhen_US
dc.contributor.authorMa, Zhimingen_US
dc.contributor.authorTriebl, Alexanderen_US
dc.contributor.authorNath, Sangeetaen_US
dc.contributor.authorCheng, Yingyingen_US
dc.contributor.authorGong, Ben-Qiangen_US
dc.contributor.authorHan, Xiaoen_US
dc.contributor.authorWang, Junqien_US
dc.contributor.authorLi, Jian-Fengen_US
dc.contributor.authorWenk, Markus R.en_US
dc.contributor.authorTorta, Federicoen_US
dc.contributor.authorMayor, Satyajiten_US
dc.contributor.authorYang, Liangen_US
dc.contributor.authorMiao, Yansongen_US
dc.date.accessioned2020-12-30T03:33:49Z-
dc.date.available2020-12-30T03:33:49Z-
dc.date.issued2020-
dc.identifier.citationTran, T. M., Ma, Z., Triebl, A., Nath, S., Cheng, Y., Gong, B.-Q., . . . Miao, Y. (2020). The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity. Life Science Alliance, 3(10), e202000720-. doi:10.26508/lsa.202000720en_US
dc.identifier.issn2575-1077en_US
dc.identifier.urihttps://hdl.handle.net/10356/145615-
dc.description.abstractQuorum sensing (QS) is a recognized phenomenon that is crucial for regulating population-related behaviors in bacteria. However, the direct specific effect of QS molecules on host biology is largely understudied. In this work, we show that the QS molecule DSF (cis-11-methyl-dodecenoic acid) produced by Xanthomonas campestris pv. campestris can suppress pathogen-associated molecular pattern–triggered immunity (PTI) in Arabidopsis thaliana, mediated by flagellin-induced activation of flagellin receptor FLS2. The DSF-mediated attenuation of innate immunity results from the alteration of FLS2 nanoclusters and endocytic internalization of plasma membrane FLS2. DSF altered the lipid profile of Arabidopsis, with a particular increase in the phytosterol species, which impairs the general endocytosis pathway mediated by clathrin and FLS2 nano-clustering on the plasma membrane. The DSF effect on receptor dynamics and host immune responses could be entirely reversed by sterol removal. Together, our results highlighted the importance of sterol homeostasis to plasma membrane organization and demonstrate a novel mechanism by which pathogenic bacteria use their communicating molecule to manipulate pathogen-associated molecular pattern–triggered host immunity.en_US
dc.description.sponsorshipNanyang Technological Universityen_US
dc.language.isoenen_US
dc.relationNIM/01/2016en_US
dc.relationM4081533en_US
dc.relation.ispartofLife Science Allianceen_US
dc.rights© 2020 Tran et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).en_US
dc.subjectScience::Biological sciencesen_US
dc.titleThe bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunityen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineering (SCELSE)en_US
dc.identifier.doi10.26508/lsa.202000720-
dc.description.versionPublished versionen_US
dc.identifier.pmid32788227-
dc.identifier.issue10en_US
dc.identifier.volume3en_US
dc.subject.keywordsQuorum Sensingen_US
dc.subject.keywordsXanthomonasen_US
dc.description.acknowledgementWe are grateful to Kimberly Kline (Singapore Centre for Environmental Life Sciences Engineering, Singapore) for critical reading of the manuscript, Yuki Nakamura (Institute of Plant and Microbial Biology, Academia Sinica, Taiwan) for valuable discussion on the lipidomics data. We thank Lay Yin Tang and Alma Turšić-Wunder for helping in RNA preparation. We thank the following researchers for sharing the Arabidopsis seeds: Takashi Ueda (National Institute for Basic Biology, Japan) for the FLS2–GFP Arabidopsis, Jianwei Pan (Lanzhou University, China) for PIN2–GFP Arabidopsis, Liwen Jiang (Chinese University of Hongkong) for the VHAa1–GFP line, and Thomas Ott (University of Freiburg) for the pREM1.2::YFP:REM1.2 line; Kathrin Schrick (Kansas State University) and Jyan-Chyun Jang (Ohio State University) for the Arabidopsis sterol mutants; and Jinbo Shen (State Key Laboratory of Subtropical Silviculture, Zhejang A&F University, China) for the BOR1–GFP line. We also thank Yinyue Deng (South China Agricultural University, China) for sharing reagents; MK Mathew and Divya Rajagopal (National Centre for Biological Sciences-TIFR, India) for helping with Arabidopsis growth for the Homo-FRET experiments. S Mayor is supported by a Margdarshi Fellowship (IA/M/15/1/502018) from the Department of Biotechnology-Wellcome Trust Alliance. This study was supported by Nanyang Technological University NIMBELS grant (NIM/01/2016) and Nanyang Technological University startup grant (M4081533) to Y Miao in Singapore.en_US
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