Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/145728
Title: Integrated transcriptomics, metabolomics, and lipidomics profiling in rat lung, blood, and serum for assessment of laser printer-emitted nanoparticle inhalation exposure-induced disease risks
Authors: Guo, Nancy Lan
Poh, Tuang Yeow
Pirela, Sandra
Farcas, Mariana T.
Chotirmall, Sanjay Haresh
Tham, Wai Kin
Adav, Sunil S.
Ye, Qing
Wei, Yongyue
Shen, Sipeng
Christiani, David C.
Ng, Kee Woei
Thomas, Treye
Qian, Yong
Demokritou, Philip
Keywords: Science::Medicine
Issue Date: 2019
Source: Guo, N. L., Poh, T. Y., Pirela, S., Farcas, M. T., Chotirmall, S. H., Tham, W. K., . . . Demokritou, P. (2019). Integrated transcriptomics, metabolomics, and lipidomics profiling in rat lung, blood, and serum for assessment of laser printer-emitted nanoparticle inhalation exposure-induced disease risks. International Journal of Molecular Sciences, 20(24), 6348-. doi:10.3390/ijms20246348
Journal: International Journal of Molecular Sciences
Abstract: Laser printer-emitted nanoparticles (PEPs) generated from toners during printing represent one of the most common types of life cycle released particulate matter from nano-enabled products. Toxicological assessment of PEPs is therefore important for occupational and consumer health protection. Our group recently reported exposure to PEPs induces adverse cardiovascular responses including hypertension and arrythmia via monitoring left ventricular pressure and electrocardiogram in rats. This study employed genome-wide mRNA and miRNA profiling in rat lung and blood integrated with metabolomics and lipidomics profiling in rat serum to identify biomarkers for assessing PEPs-induced disease risks. Whole-body inhalation of PEPs perturbed transcriptional activities associated with cardiovascular dysfunction, metabolic syndrome, and neural disorders at every observed time point in both rat lung and blood during the 21 days of exposure. Furthermore, the systematic analysis revealed PEPs-induced transcriptomic changes linking to other disease risks in rats, including diabetes, congenital defects, auto-recessive disorders, physical deformation, and carcinogenesis. The results were also confirmed with global metabolomics profiling in rat serum. Among the validated metabolites and lipids, linoleic acid, arachidonic acid, docosahexanoic acid, and histidine showed significant variation in PEPs-exposed rat serum. Overall, the identified PEPs-induced dysregulated genes, molecular pathways and functions, and miRNA-mediated transcriptional activities provide important insights into the disease mechanisms. The discovered important mRNAs, miRNAs, lipids and metabolites may serve as candidate biomarkers for future occupational and medical surveillance studies. To the best of our knowledge, this is the first study systematically integrating in vivo, transcriptomics, metabolomics, and lipidomics to assess PEPs inhalation exposure-induced disease risks using a rat model.
URI: https://hdl.handle.net/10356/145728
ISSN: 1661-6596
DOI: 10.3390/ijms20246348
Rights: © 2020 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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