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Title: Flavivirus cross-reactivity to dengue nonstructural protein 1 antigen detection assays
Authors: Tan, Li Kiang
Wong, Wing Yan
Yang, Hui Ting
Huber, Roland G.
Bond, Peter J.
Ng, Lee Ching
Maurer-Stroh, Sebastian
Hapuarachchi, Hapuarachchige Chanditha
Keywords: Science::Medicine
Issue Date: 2019
Source: Tan, L. K., Wong, W. Y., Yang, H. T., Huber, R. G., Bond, P. J., Ng, L. C., . . . Hapuarachchi, H. C. (2019). Flavivirus cross-reactivity to dengue nonstructural protein 1 antigen detection assays . Diagnostics, 10(1), 11-. doi:10.3390/diagnostics10010011
Project: H1699f0013
Journal: Diagnostics
Abstract: Dengue virus (DENV) and Zika virus (ZIKV) are flaviviruses of public health relevance. Both viruses circulate in the same endemic settings and acute infections generally manifest similar symptoms. This highlights the importance of accurate diagnosis for clinical management and outbreak control. One of the commonly used acute diagnostic markers for flaviviruses is nonstructural protein 1 (NS1). However, false positives due to antigenic cross-reactivity have been reported between DENV and ZIKV infections when using DENV NS1 antigen (NS1 Ag) detection assays in acute cases. Therefore, we investigated the lowest detectable virus titres and cross-reactivity of three commercial dengue NS1 Ag rapid assays and two ELISAs for different flaviviruses. Our results showed that substantially high viral titres of ZIKV, Kunjin virus (KUNV) and yellow fever virus (YFV) are required to give false-positive results when using DENV NS1 rapid detection assays. Commercial DENV NS1 ELISAs did not react with ZIKV and YFV. In comparison, tested assays detected DENV at a significantly low virus titre. Given the relatively low viral loads reported in clinical samples, our findings suggest that commercially available dengue NS1 Ag detection assays are less likely to generate false-positive results among clinical samples in areas where multiple flaviviruses cocirculate.
ISSN: 2075-4418
DOI: 10.3390/diagnostics10010011
Rights: © 2019 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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