Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/145937
Title: Inclusion of cross-linked elastin in gelatin/PEG hydrogels favourably influences fibroblast phenotype
Authors: Cao, Ye
Lee, Bae Hoon
Irvine, Scott Alexander
Wong, Yee Shan
Peled, Havazelet Bianco
Venkatraman, Subramanian
Keywords: Engineering::Materials
Issue Date: 2020
Source: Cao, Y., Lee, B. H., Irvine, S. A., Wong, Y. S., Peled, H. B., & Venkatraman, S. (2020). Inclusion of cross-linked elastin in gelatin/PEG hydrogels favourably influences fibroblast phenotype. Polymers, 12(3), 670-. doi:10.3390/polym12030670
Journal: Polymers
Abstract: The capacity of a biomaterial to innately modulate cell behavior while meeting the mechanical property requirements of the implant is a much sought-after goal within bioengineering. Here we covalently incorporate soluble elastin into a gelatin–poly (ethylene glycol) (PEG) hydrogel for three-dimensional (3D) cell encapsulation to achieve these properties. The inclusion of elastin into a previously optimized gelatin–PEG hydrogel was then evaluated for effects on entrapped fibroblasts, with the aim to assess the hydrogel as an extracellular matrix (ECM)-mimicking 3D microenvironment for cellular guidance. Soluble elastin was incorporated both physically and covalently into novel gelatin/elastin hybrid PEG hydrogels with the aim to harness the cellular interactivity and mechanical tunability of both elastin and gelatin. This design allowed us to assess the benefits of elastin-containing hydrogels in guiding fibroblast activity for evaluation as a potential dermal replacement. It was found that a gelatin–PEG hydrogel with covalently conjugated elastin, supported neonatal fibroblast viability, promoted their proliferation from 7.3% to 13.5% and guided their behavior. The expression of collagen alpha-1(COL1A1) and elastin in gelatin/elastin hybrid gels increased 16-fold and 6-fold compared to control sample at day 9, respectively. Moreover, cells can be loaded into the hydrogel precursor solution, deposited, and the matrix cross-linked without affecting the incorporated cells adversely, thus enabling a potential injectable system for dermal wound healing.
URI: https://hdl.handle.net/10356/145937
ISSN: 2073-4360
DOI: 10.3390/polym12030670
Rights: © 2020 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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