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Title: Gene profiles in the early stage of neuronal differentiation of mouse bone marrow stromal cells induced by basic fibroblast growth factor
Authors: Yu, Lili
Hong, Wei
Yang, Haijie
Xia, Yin Yan
Feng, Zhiwei
Keywords: Science::Biological sciences
Issue Date: 2020
Source: Yu, L., Hong, W., Yang, H., Xia, Y. Y., & Feng, Z. (2020). Gene profiles in the early stage of neuronal differentiation of mouse bone marrow stromal cells induced by basic fibroblast growth factor. Stem Cells International, 2020, 8857057-. doi:10.1155/2020/8857057
Journal: Stem Cells International
Abstract: A stably established population of mouse bone marrow stromal cells (BMSCs) with self-renewal and multilineage differentiation potential was expanded in vitro for more than 50 passages. These cells express high levels of mesenchymal stem cell markers and can be differentiated into adipogenic, chondrogenic, and osteogenic lineages in vitro. Subjected to basic fibroblast growth factor (bFGF) treatment, a typical neuronal phenotype was induced in these cells, as supported by neuronal morphology, induction of neuronal markers, and relevant electrophysiological excitability. To identify the genes regulating neuronal differentiation, cDNA microarray analysis was conducted using mRNAs isolated from cells differentiated for different time periods (0, 4, 24, and 72 h) after bFGF treatment. Various expression patterns of neuronal genes were stimulated by bFGF. These gene profiles were shown to be involved in developmental, functional, and structural integration of the nervous system. The expression of representative genes stimulated by bFGF in each group was verified by RT-PCR. Amongst proneural genes, the mammalian achate-schute homolog 1 (Mash-1), a basic helix-loop-helix transcriptional factor, was further demonstrated to be significantly upregulated. Overexpression of Mash-1 in mouse BMSCs was shown to induce the expression of neuronal specific enolase (NSE) and terminal neuronal morphology, suggesting that Mash-1 plays an important role in the induction of neuronal differentiation of mouse BMSCs.
ISSN: 1687-966X
DOI: 10.1155/2020/8857057
Rights: © 2020 Lili Yu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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