Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/146217
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dc.contributor.authorYu, Lilien_US
dc.contributor.authorHong, Weien_US
dc.contributor.authorYang, Haijieen_US
dc.contributor.authorXia, Yin Yanen_US
dc.contributor.authorFeng, Zhiweien_US
dc.date.accessioned2021-02-02T07:23:46Z-
dc.date.available2021-02-02T07:23:46Z-
dc.date.issued2020-
dc.identifier.citationYu, L., Hong, W., Yang, H., Xia, Y. Y., & Feng, Z. (2020). Gene profiles in the early stage of neuronal differentiation of mouse bone marrow stromal cells induced by basic fibroblast growth factor. Stem Cells International, 2020, 8857057-. doi:10.1155/2020/8857057en_US
dc.identifier.issn1687-966Xen_US
dc.identifier.other0000-0001-9314-895X-
dc.identifier.urihttps://hdl.handle.net/10356/146217-
dc.description.abstractA stably established population of mouse bone marrow stromal cells (BMSCs) with self-renewal and multilineage differentiation potential was expanded in vitro for more than 50 passages. These cells express high levels of mesenchymal stem cell markers and can be differentiated into adipogenic, chondrogenic, and osteogenic lineages in vitro. Subjected to basic fibroblast growth factor (bFGF) treatment, a typical neuronal phenotype was induced in these cells, as supported by neuronal morphology, induction of neuronal markers, and relevant electrophysiological excitability. To identify the genes regulating neuronal differentiation, cDNA microarray analysis was conducted using mRNAs isolated from cells differentiated for different time periods (0, 4, 24, and 72 h) after bFGF treatment. Various expression patterns of neuronal genes were stimulated by bFGF. These gene profiles were shown to be involved in developmental, functional, and structural integration of the nervous system. The expression of representative genes stimulated by bFGF in each group was verified by RT-PCR. Amongst proneural genes, the mammalian achate-schute homolog 1 (Mash-1), a basic helix-loop-helix transcriptional factor, was further demonstrated to be significantly upregulated. Overexpression of Mash-1 in mouse BMSCs was shown to induce the expression of neuronal specific enolase (NSE) and terminal neuronal morphology, suggesting that Mash-1 plays an important role in the induction of neuronal differentiation of mouse BMSCs.en_US
dc.language.isoenen_US
dc.relation.ispartofStem Cells Internationalen_US
dc.rights© 2020 Lili Yu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleGene profiles in the early stage of neuronal differentiation of mouse bone marrow stromal cells induced by basic fibroblast growth factoren_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doi10.1155/2020/8857057-
dc.description.versionPublished versionen_US
dc.identifier.pmid33424980-
dc.identifier.scopus2-s2.0-85098698834-
dc.identifier.volume2020en_US
dc.subject.keywordsBone Marrow Stromal Cellsen_US
dc.subject.keywordsFactoren_US
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item.grantfulltextopen-
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